McGuirl, Michele A.

English

University of Montana

University of Montana ScholarWorks at University of Montana Syllabi Course Syllabi 1-2003 BIOC 486.01: Biochemistry Michele A. McGuirl University of Montana - Missoula, michele.mcguirl@umontana.edu Follow this and additional works at: https://scholarworks.umt.edu/syllabi Let us know how access to this document benefits you. Recommended Citation McGuirl, Michele A., "BIOC 486.01: Biochemistry" (2003). Syllabi. 1180. https://scholarworks.umt.edu/syllabi/1180 This Syllabus is brought to you for free and open access by the Course Syllabi at ScholarWorks at University of Montana. It has been accepted for inclusion in Syllabi by an authorized administrator of ScholarWorks at University of Montana. For more information, please contact scholarworks@mso.umt.edu. Biochemistry 486  Putting 4 Years of Theory to Work!  Learn molecular biology and biochemical techniques!  Lab and lecture are integrated into a semester-long research project.  §      Site-directed mutagenesis §      Recombinant protein expression in bacteria §      PCR techniques §      Protein purification §      Protein characterization §      SDS-PolyAcrylamide Gel Electrophoresis §      UV/Vis Spectroscopy §      Electrochemistry §      Electron Paramagnetic Resonance  Enrollment is limited, so sign up soon!        BIOC 486        CRN 33040      Currently 2 credits - might be 3 by 2003      McGuirl      TR      9:30A-12:30P      HS 406              The main goal is to understand how mutations in amino acid sequence may affect the biochemical properties of a protein. Each student will prepare a different mutant of azurin, a blue copper protein that is involved in electron transfer during bacterial respiration. The mutants will be purified and characterized by a variety of spectroscopic techniques. At the end of the course, students will compare notes and write a summary of the effects of the various mutations on the biochemical properties of the protein. Students will gain experience in gene cloning and recombinant protein expression in bacteria, site-directed mutagenesis, PCR techniques, protein purification, and protein characterization, including several types of spectroscopy. They will also be given at least one journal article that we will discuss in class. 

BIOC 486.01: Biochemistry

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BIOC 486.01: Biochemistry

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