When a gene or an  unidentified fragment of DNA from an organism has been cloned, it is often useful to map its site of chromosomal origin with respect  to known   markers

Grotelueschen, J.

Metzenberg, R. L.

English

Kansas State University

Fungal Genetics Reports Volume 35 Article 17 Restriction polymorphism maps of Neurospora crassa: updates R. L. Metzenberg J. Grotelueschen Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Metzenberg, R. L., and J. Grotelueschen (1988) "Restriction polymorphism maps of Neurospora crassa: updates," Fungal Genetics Reports: Vol. 35, Article 17. https://doi.org/10.4148/1941-4765.1537 This Regular Paper is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact cads@k-state.edu. Restriction polymorphism maps of Neurospora crassa: updates Abstract When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful to map its site of chromosomal origin with respect to known markers. This regular paper is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol35/iss1/17 Metzenberg, R.L. and J. GrotelueschenRestriction polymorphism maps ofNeurospora  crassa: updatesWhen a gene or an unidentified fragment of DNAfrom an organism has been cloned, it is oftenuseful to map its site of chromosomal origin withrespect to known markers.   The methods andmaterials for doing this, and some data onsegregation of markers, has been published(Metzenberg  et  al. 1984.  Neurospora Newsl.  31:35-39; ibid. Proc. Natl. Acad. Sci. U.S.1985. 82:2067-2071; Metzenberg and Grotelueschen, 1987. Fungal Genetics Newsl. 34:39-44). The following data include the previous scorings of two crosses from the 1987article and contains new data on the same two crosses from our own lab, and from others.As noted in the 1987 article, 38 segregants from the first cross were taken from orderedasci, and provide somewhat more information than can be obtained from the 18 segregantswhich represent random spores from the second cross. Both crosses, however, have beenused in  a number of laboratories, and data from both are presented.  The scoring ofsegregants is coded in the same way as before: "M" or "0" indicate segregants that arelike the Mauriceville parent or like the Oak-Ridge-derived parent, respectively:  "-"indicates that the scoring was not done or was equivocal for technical reasons; and (O)in Isolate I and (M) in Isolate 6 for all lanes of the second cross means that these arenot progeny but are the parental strains of the cross, and are 0 and M by definition.The notation for genes or DNA fragments mapped in these crosses is a mixed one.  Asbefore, some are obvious gene symbols (e.g. thi-4) and are indexed in the compendium ofloci (Perkins et al. 1982.  Microbiol. Rev. 46:426-570).   Those with simple numbers like33 or 1 or 18, unprefaced by zeros, are the loci of 5S rDNAs, as in the 1984 article.Those containing a colon (e.g. 12:8B) are loci identified by probing blots with thecorresponding cosmid from the Vollmer-Yanofsky clonal library (Vollmer and Yanofsky1986.  Proc. Natl. Acad Sci. U.S.  83:4869-4873).  H3H4 is histone H3 + H4 (Woudt et al.1983.  Nucleic Acids Res.  11:5347-5360).  con loci are associated with conidiation(Berlin and Yanofsky 1985. Molec. Cell. Biol. 5:839-848; ibid. 849-855).  Loci withnames starting with LZ and DB are arbitrary DNA fragments of unknown function, studied inour laboratory by Ludwika Zagorska and David Butler, respectively.   hbs is "homebase",studied in J. Kinsey's laboratory.  cat-2 (Simmons et al. 1987.  Fungal Genetics Newsl.34:55-56) is a catalase-encoding gene scored by protein polymorphism rather than DNApolymorphism; note that its segregation in Ascus E suggests the occurrence of a geneconversion. Finally, the substantial number of loci whose numbers begin with one or morezeros are data that have been reported to us, but whose authors would like the loci toremain unidentified and themselves to be anonymous until publication or five years haveelapsed, whichever is first. Even without identification, the results enrich the map andhelp others map their clones to a chromosome.If you have found these data useful please pass on the favor by pencilling any results ofyour own, including those from random fragments and from "mistakes" in cloning onto acopy of the appropriate page from this article and sending it to RLM.  You may ask that anumber which preserves confidentiality be assigned to it, or if you are willing for thegene and yourself to be identified, that will be done. If we cooperate on this, we canhope to see this map become more densely and increasingly useful.  Supported by NIH GrantGM 08995. - - - Department of Physiological Chemistry, University of Wisconsin, Madison,WI 53706

Restriction polymorphism maps of Neurospora crassa: updates

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Restriction polymorphism maps of Neurospora crassa: updates

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