Response of two loci to interaction treatmen

Auerbach, C.

Kilbey, B. J.

Kolmark, G.

Fungal Genetics Reports

English

C. Auerbach

B. J. Kilbey

G. Kolmark

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Response of two loci to interaction treatment

Kansas State University

Fungal Genetics Reports Volume 2 Article 2 Response of two loci to interaction treatment C. Auerbach B. J. Kilbey G. Kolmark Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Auerbach, C., B.J. Kilbey, and G. Kolmark (1962) "Response of two loci to interaction treatment," Fungal Genetics Reports: Vol. 2, Article 2. https://doi.org/10.4148/1941-4765.1041 This Research Note is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact cads@k-state.edu. Response of two loci to interaction treatment Abstract Response of two loci to interaction treatment This research note is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol2/iss1/2 Auerbach, C., B. J. Kilbeyond G. Kplmark Response of two loci to inter- action treatment. RESEARCH NOTES The introduction into the cell of a mutagenic agent and the emergence of CI mutant colony clre connected by CI pathway which includes, among other processes, stabilization or decay of potential mutations and the successful emergence of the mutant phenotype. Most mutagens, in addition to initiating changes in DNA, clre likely to affect some of these ancillary processes. Successive treatments with two mutagens can be used for studying how one of them affects the mutagenic pathway of the other. Experiments of this kind, started several years ago in Oak Ridge (Kfilmark 8. Auerbach, MGB. l7) and now resumed, suggest that in the doubly auxotrophic strain K3/17ad-3A (38701); ~nos (37401) the mutagenic pathways for reverse mutations at the two loci differ. The results on which this conclusion is based clre shown qualitatively in the table, in which DEB stands for diepoxybutane, + for more than additive, -for less than additive. The two cases noted as “variable” clre under analysis; possibly variability is correlated with the relative strengths of the two treatments. No. of expts. Successive treatments &-reversions &-reversions DEB + UV UV + DEB + variable (never +) variable I HN02 + UV + UV+ HNO, + Mailing et al. (Int. J. Rad. Biol. 4, 328) obtained similar results with interaction treatment of the sclme strain by UV and CH 0. At present, our analysis aims at determining at what points in the mutagenic pathways these interc?ctions take place. The two loci differ strikingly in their patterns of mutagen specificity. Our results suggest that, at least in part, this may be a reflection of differences in mutagenic pathways. --- Mutagenesis Research Unit, Institui% of Animal Genetics, Edinburgh, Scotland. Auerbach, C., B. J. Kilbeyand G. In the doubly ouxotrophic strain K3/17 & (38701) Kblmark. Differences in dose-effect im (37401), treatment with moderate doses of UV curves for UV-induced reverse mutations usually yields about twice as many &-reversions as at two different loci. d-reversions, and this has been considered as evidence for CI difference in UV-sensitivity between the two loci or sites. Mutoticm tests with low doses of UV have shown this to be an oversimplified interpretation, the difference between the loci being one of dose-effect curve rather than of general sensitivity to UV-treat- merit. While the curve for &-reversions rises steeply with dose, that for&reversions is flatter and levels off at intermediate doses. The table shows the ratio of &-reversions to ~-reversions in 3 experiments in which the dose was controlled by exposure time. Since the number of spores was not the same in all experiments and series, these ratios are not strictly comparable, but the increase within each experiment and in the mean values is consistent and clear. 

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Response of two loci to interaction treatment

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