A method for storing conidia of Neurospor

Mücke, D.

English

Kansas State University

Fungal Genetics Reports Volume 2 Article 22 A method for storing conidia of Neurospora D. Mücke Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Mücke, D. (1962) "A method for storing conidia of Neurospora," Fungal Genetics Reports: Vol. 2, Article 22. https://doi.org/10.4148/1941-4765.1061 This Technical Note is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact cads@k-state.edu. A method for storing conidia of Neurospora Abstract A method for storing conidia of Neurospora This technical note is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol2/iss1/22 Mitchell, M. EL Storage of culture Polyethylene bogs of the sort sold for home freezing of medio in polyethylene bogs. foods have been found very convenient for packaging agor slants, agar plates, or even tubes of liquid media, which ore kept on hand, stored under refrigeration. No rock or other support is needed for tubes. They may simply be packed into the bags whose tops ore then secured. Thus refrigerator space is conserved and evaporation of water from media is much more effectively retarded than by wrappings of foil or waxed paper. The bags moy be rewsed repeatedly. Petri dishes should be placed upright and tubes should be plugged with non-absorbent cotton to prevent contaminants from being tarried inside the vessels by con- densed moisture which has been in contact with external surfaces. ---Biology Division, California Institute of Technology, Pasadena, California. Mi;cke, D. A method for storing 2. 84 g Na2SOq (anhydrous) ore stirred with I. 2 ml of conidia ofNeurospora. an enriched conidia-suspension. The pulp of crystals is then stored under sterile conditions at +4’C. For use (I complete Neurospom culturemedium is inoculated with some of the crystals and incubated at 25’C. Viability was good for o period of more than 3 yews, maintaining the original biological qualities of the Neurospora strains (wild-type; biochemical mutant strains). ---Physiological Chemistry Institute, University of Restock, Restock, Germany. Pittenger, T.H. Special growth tubes When o auxotrophic mutant such os & is used in for the study of transport of growth certain incompatible heterokaryotic combinations, the factors and other phenomena. nit-2 strain moy grow for long distances in the growth tube before ceasing to grow. This is not observed with nit-2 homokaryons under similar conditions. This growth, except at the very proximal end of the tube where o mixture of conidia has been placed, appears from certain criteria, except growth on minimal, to be homokaryotic. This is assumed not only because of the dark brown accumulation in the media charac- teristic of this mutant when grown on submaximal amounts of the growth factor but also because conidial plating foils to disclose the presence of anything except the nit-2 strain. Such observations prompted o reexamination of the studies of Ryan et al., Am. J. Bat. 3: 784, 1943 concerning the extent of the tronslocotion of various compounds in the mycelium of Neurospom. For this purpose o special type of growth tube, on improved design of one originally used by Pittenger and Atwood, was constructed; sub- sequently such tubes have been found to be equally useful in other experiments. With the hope that others may find it adaptable to other problems, o brief description of the growth tube is presented, along with some preliminary results illustrating its use. This growth tube was originally designed to help determine indirectly whether o mycelium of an auxotrophic mutant growing on supplemented medium could tronslocote enough of the essential growth factor from such o medium to enable the organism to continue,to grow for an extended time, on minimal medium. The growth tube consists of two 20 mm. diameter pyrex glass tubes united by o 24/40 standard taper ground glass interchangeable joint (Corning No. 6540). The distal end of the inter portion of the ground glass joint is partially sealed to provide o partition between the two sections of the tube when the joints ore united. The glass partition prevents contact of the media present in the proximal and distal ends of the growth tube, but the small horizontal opening in the upper portion of the partition permits the mycelium to pass readily from one section of the tube to the other. To facilitate sampling of conidia and for aeration, the tubes ore equipped with sampling ports along the upper surface. These are mode of IO mm. diameter tubing, 30 mm. high and ore spaced at 4-6 inch intervals along the length of the tube. The pro- ximal portion of the tube is approximately nine inches long and the distal portion, 20 inches long, but length moy vary with different types of experiments. The opposite ends of the finished tube ore bent at o 45 angle. Observations relating to the efficiency of transport to growth factors essential to auxotrophic mutant strains can be mode in such tubes. The growth factor required by the mutant strain is added to the proxi- mal section of the tube, along with 3% agar solidified Fries medium and 1.5% sucrose. To the distal par- tion of the tube is added the sane medium without the growth factor. The glass partition prevents the 

A method for storing conidia of Neurospora

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A method for storing conidia of Neurospora

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