In this work, 1.8 nm graphene quantum dots (GQDs), exhibiting bright blue fluorescence, were prepared using a bottom-up synthesis from citric acid. The fluorescence of the GQDs could be almost completely quenched (about 96%) by adding Hg2+. Quenching was far less efficient with other similar heavy metals, Tl+, Pb2+ and Bi3+. Fluorescence could be near quantitatively restored through the introduction of thiocyanate. This “turn-on” fluorescence can thus be used to detect both or either environmental and physiological contaminants mercury and thiocyanate and could prove useful for the development of simple point-of-care diagnostics in the future. [Figure not available: see fulltext.]