Adenot, Pierre,

Aguirre-Lavin, Tiphaine,

Araujo, Thamiris Dornelas

Beaujean, Nathalie,

Camargo, Luiz Sergio Almeida

Louro, Iuri Drumond

Mendes, Vivian Rachel Araujo

Souza, Eliza Diniz

International audienceHeat stress compromises bovine oocyte developmental competence, but the effects of high temperature during oocyte maturation on embryo chromatin organization is unknown. In this study bovine oocytes were exposed to heat shock (41°C) for 12 h during in vitro maturation and then submitted to in vitro fertilization. The heat shock did not affect (P > 0.05) the cleavage but reduced (P  0.05) on total cell number was found, but the heat shock increased (P < 0.05) the proportion of apoptotic cells in blastocysts at Day 8. Immunofluorescence analysis of H3K9me3 and HP1 was performed in embryos at 52 h post in vitro fertilization. An accumulation of H3K9me3 in the nuclei of embryos derived from heat-shocked oocytes at four-cell and eight-cell stages was found. Also, a non-expected higher proportion (P < 0.05) of four-cell stage embryos displaying nuclei with increased HP1 fluorescence was observed, suggesting an abnormal chromatin compaction in embryos from heat-shocked oocytes. Embryos at eight-cell stage derived from heat-shocked oocytes displayed lower (P < 0.05) relative amount of HSP40 transcripts than control ones. In conclusion, heat shock before fertilization has an effect on embryo chromatin, influencing the accumulation of H3K9me3 and HP1 in early embryos as well as further development

Aguirre-Lavin, Tiphaine

Adenot, Pierre

Beaujean, Nathalie

Hal-Diderot

Heat shock during in vitro maturation induces chromatin modifications in the bovine embryo

Archive Ouverte en Sciences de l'Information et de la Communication

Heat shock during in vitro maturation induces chromatin modifications in the bovine embryo

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Hal-Diderot

Archive Ouverte en Sciences de l'Information et de la Communication