Conté: Part 1, General vision and presentation of the plant / Ferran Vila. Part 2, Technical characteristics of the process / Carla Sánchez. Part 3, Analysis and future perspective / Ramon Roc

Roca, Ramon

Sánchez, Carla

Universitat Autònoma de Barcelona. Facultat de Biociències

Vila, Ferran

Diposit Digital de Documents de la UAB

Design of a recombinant tissue plasminogen activator production plantGENERAL VISION AND PRESENTATION OF THE PLANTAuthors: Carla Sánchez, Ramon Roca i Ferran Vila Biotechnology Final Project, 2013Tutor: Prof. Carles Solà .                  Universitat Autònoma de BarcelonaINTRODUCTIONTPA•Tissue plasminogen activator(tPA) is a serine protease involved inthe breakdown of the blood clots.•Tenecteplase (TNK) is a recombinant variant of the tPA, which hashigher fibrin specificity and greater resistance to inactivation.Figure 1. Crystalstructure of tPAFigure 2. The bloodclotting cascadewhere acts tPAOBJECTIVE OF THE PROJECTPRIMITIVE PROBLEM: Design a process for producing tPA inChina.SPECIFIC PROBLEM: Produce 30 kg / year of TNK, which coverabout 5% of cases of cerebrovascular disease China.MATERIALS AND METHODSSuperPro Designer is a simulator that facilitates the planning,modeling, evaluation and optimization of processes. Once thesearch for information, knowing the equipment, the operationsthat are carried out in each one and the inputs and outputs, wecan make balances and economical calculations.1•Approved by Food and Drug Adminsitration (FDA) in the year2000.•It is used for treating cerebrovascular diseases caused by bloodclots.DESCRIPTION OF THE SOLUTIONBlocks diagramFlow diagramP&I Diagram Lay-out•MAIN CHARACTERISTICS OF THE PROCESS•Continuous operation, operating for three months. Perfusion.•CHO cell line DUKxB11. Immobilized cells.•Production of 30 kg / year of TNK.Autodesk AutoCAD is a computer-aided design software fordrawing in two and three dimensions. Having defined the flowwill allow us design the layout.PROCESS SUMMARYThe process consists of three parts clearly separated:Upstream (Blue). Preparation of raw materials and inoculum. Duration: 10 days.Reaction (Red). In continuous with immobilized cells. Medium flow: 500 L/h.Downstream (Black). Separation and purification of the product of interest.The technical characteristics of the process are specifically addressed in the second poster.ECONOMICAL SUMMARYThe comprehensive economic analysis is the third poster.Capital Investment Charged to This Project 1 039 226 000 $Payback Time 2.58 yearsUnit Production Cost 1 461 403.06 $/kgUnit Production Revenue 25 000 000 $/kgital Investment Charged to This Project 039 millions $ack Time  yearsit roduction Cost .  millions $/kgit roduction Revenue  millions $/kg6PRESENTATION OF THE PLANTWe have an area of 50.000 m2 in ChinaDifferent areas depending on if requiredsterility:•Offices (Red)•Production area (green)•All entries in this area should be controlled.Everything in and out of there must have beenpreviously sterilized, maintaining theseconditions at all times.•Truck’s circuit (blue)•We thought it appropriate to make a separateentry and exit of trucks to ease traffic andminimize maneuvering in and out of the docks.•Parking for employees and visitors.The entire facility is fenced and all inputs areguarded.1. Checkpoints1. Input from outsideand dressing rooms2. Laboratory3. Toilets4. Production area5. Store of the rawmaterials6. Store of the finalproduct7. Liquid storagetanks1. Toilets2. Offices3. Auditorium4. Offices andmeeting rooms5. Cafeteria6. Input from outside7. Input from theparkingSUMMARY AND CONCLUSIONS REFERENCESGennova biopharmaceuticals. (2012). WO 2012/085933 A1. World Intellectual Property Organization.For the query of most commodity prices: Alibaba Portal. Available on the website: www.alibaba.com/Prices media used: Invitrogen catalog. Available on the web: https://products.invitrogen.com/45127We have built a plausible flux diagram, and a possiblelay-out of a plant to produce TNK. Although it is anapproach, it seems to be an economically viableproject.

Design of a recombinant tissue plasminogen activator production plant

http://ddd.uab.cat/pub/tfg/2013/116060/TFG_ferranvilajulia.pdf.pdf

Design of a recombinant tissue plasminogen activator production plant

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