Expanding nature’s catalytic repertoire to include reactions important in synthetic chemistry will open new
opportunities for ‘green’ chemistry and biosynthesis. We demonstrate the first enzyme-catalyzed insertion
of carbenoids into N–H bonds. This type of bond disconnection, which has no counterpart in nature, can be
mediated by variants of the cytochrome P450 from Bacillus megaterium. The N–H insertion reaction takes
place in water, provides the desired products in 26–83% yield, forms the single addition product exclusively,
and does not require slow addition of the diazo component
