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research
Genetic engineering of the β-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds
Authors
Mario Aguedo
J.-M. Belin
+6 more
E. E. Garcia
A. Groguenin
F. Husson
M.-T. LeDall
J.-M. Nicaud
Y. Waché
Publication date
1 June 2003
Publisher
'Elsevier BV'
Doi
Cite
Abstract
peer reviewedThe yeast Yarrowia lipolytica possesses five acyl-CoA oxidases (Aox1p to 5), the enzyme catalysing the first reaction of β-oxidation. The understanding of the specific role of each acyl-CoA oxidase is important to construct a yeast strain growing at a good rate and able to produce without degrading the aroma compound γ-decalactone. In this study we observed that Aox4p exhibits a slight activity on a broad spectrum of substrates and that it is involved in lactone degradation. We constructed a strain lacking this activity. Its growth was only slightly altered and it produced 10 times more lactone than the wild type in 48h. © 2004 Elsevier B.V. All rights reserved
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oai:HAL:hal-02680321v1
Last time updated on 08/06/2020
Open Repository and Bibliography - Liège
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oai:orbi.ulg.ac.be:2268/162763
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Open Repository and Bibliography - Liège
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Last time updated on 25/02/2014