Recombinant protein expression in insect cell systems

Abstract

Since the introduction of baculovirus expression vectors, the suitability of insect cells for the expression of functionally active genes of eukaryotic origin has been exhaustively documented. Originally realization of a functional viral expression vector was laborious and depended on double homologous in vivo recombination success as well as successful purification of recombinant baculovirus by plaque assay. Using the commercial Bac to Bac(TM) expression system we confirm that transposon-assisted recombination and cloning of recombinant transfectable bacmid DNA in bacteria now allow fast productive expression of a gene of interest.status: publishe