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乙二醇二缩水甘油醚交联血红蛋白的反应优化
Authors
徐宇红
苏志国
+3 more
谭天伟
路秀玲
郑春杨
Publication date
1 January 2006
Publisher
Abstract
将乙二醇二缩水甘油醚(EGDE)作为血红蛋白(Hb)的交联剂制备红细胞代用品.采用SDS-PAGE电泳、高效液相色谱和多角度激光散射检测器联用鉴定交联产物.实验表明,在pH 8.7,血红蛋白浓度为20 mg·mL^-1,修饰剂EGDE与血红蛋白的摩尔比达100:1时反应4 h,交联产物中90%为分子内交联的血红蛋白.在pH 7.5、血红蛋白浓度为200mg·mL^-1,修饰剂EGDE与血红蛋白的摩尔比为25:1时反应12 h,交联产物主要为寡聚血红蛋白,分子量范围在145-375 kD之间;其中二聚体血红蛋白占总蛋白量的74.7%,三聚体血红蛋白18.9%,四到六聚血红蛋白6.4%,几乎无高聚物生成.采用血氧分析仪对交联产物进行活性鉴定,制备的分子内交联和寡聚血红蛋白均具有携氧能力,小鼠的生物学实验表明产品无异常毒性,具备了作为红细胞代用品的可能性
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
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oai:ir.ipe.ac.cn:122111/47173
Last time updated on 11/04/2021
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
See this paper in CORE
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oai:ir.ipe.ac.cn:122111/36989
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Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
See this paper in CORE
Go to the repository landing page
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oai:ir.ipe.ac.cn:122111/36991
Last time updated on 05/12/2019