Amato, Letizia

Boisen, Anja

Caviglia, Claudia

Dimaki, Maria

Emnéus, Jenny

Gorton, Lo

Heiskanen, Arto

Keller, Stephan Sylvest

Ortiz, Roberto

Svendsen, Winnie Edith

Tenje, Maria

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 17, 2017Electrochemical evaluation of dopamine detection on pyrolysed carbon and goldelectrodesAmato, Letizia; Heiskanen, Arto; Caviglia, Claudia; Tenje, Maria; Dimaki, Maria; Svendsen, Winnie Edith;Ortiz, Roberto; Gorton, Lo; Keller, Stephan Sylvest; Boisen, Anja; Emnéus, JennyPublication date:2012Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Amato, L., Heiskanen, A., Caviglia, C., Tenje, M., Dimaki, M., Svendsen, W. E., ... Emnéus, J. (2012).Electrochemical evaluation of dopamine detection on pyrolysed carbon and gold electrodes. Poster sessionpresented at 63rd Annual Meeting of the International Society of Electrochemistry, Prague, Czech Republic.El t h i l E l ti f D i D t tiec roc em ca va ua on o opam ne e ec on on           Pyrolysed Carbon and Gold Electrodes     Letizia Amato1 Arto Heiskanen1 Claudia Caviglia1 Maria Tenje2 Maria Dimaki1 Winnie Svendsen1 Roberto Ortiz3  ,    ,    ,    ,    ,    ,    , Lo Gorton3 Stephan S Keller1 Anja Boisen1 Jenny Emnéus1  ,    .  ,    ,   1 Technical University of Denmark Department of Micro‐ and Nanotechnology Denmark; 2 Department of Measurement Technology and      ,        ,             3 fIndustrial Electrical Engineering, Lund University, Sweden;   Center  or Molecular Protein Science, Lund University, SwedenCarbon electrodes have been widely used as electrode material in dopamine detection [1]. To obtain arrays of microelectrode structures, gold hasbeen a preferable material due to easy lithographic fabrication techniques [2].Gold electrodes need however a modification e g thiol SAM [3] to eliminate the effect of fouling in longer measurements of dopamine, , , . . , .•We characterized the electrochemical behaviour of interdigitated electrodes (IDE) of pyrolysed carbon (SU‐8 patterned structures) and gold•We tested the materials based on the characteristics of dopamine detection.The CVs (2nd cycle) in the figure below show that for carbon theThe electrochemical and materials properties of gold (fig A) and peaks are well‐defined and the peak currents are slightly higher..Additi ll th h t i ti l t d f li d bpyrolysed carbon IDEs (fig. B and C) were characterized using cyclic ona y, e c arac er s c e ec ro e ou ng cause ylt t d l t h i l i d t (EIS) dopamine deposition during continued detection [3] is lessvo amme ry an e ec roc em ca mpe ance spec roscopy .pronounced on carbon.A BC li l f 5 Myc c vo tammograms o mdopamine in PBS (pH 7) on gold(black) and pyrolysed carbon (red)(th 2nd CV i h f he s s own or eacmaterial; potentials vs. Ag/AgClO ti l i i i f RE and sweep rate: 50 mV/s)p ca m croscop c mages oi di i d l d (IDE )C.nter g tate e ectro es s :( ) b d ldA e‐ eam evaporate god ( ) l d b fan B pyro yse car on romthe negative photoresist SU‐8 .C. SEM image of carbon IDEs. The carbon electrode surface was electrochemically modified withb li b di i lt h i t Th difi ticar oxy c groups y azon um sa c em s ry. e mo ca onresulted in lower response (fig A) but interestingly the peak. ,CARBON GOLD current ratio increased toward unity and facilitated the electrodet bili ti ft 5 l Thi h b d t thC li lt t diff ts a za on a er cyc es. s p enomenon can e ue o eyc c vo ammograms a erenclose vicinity of the electroactive group in dopamine (OH group)scan rates of 1 mM rutheniumhexamine chloride (II/III) in PBS with the carboxylic acid, leading to faster kinetics of dopamine(pH 7) on IDE of pyrolysed id ti (l Δ ) t th b li id difi d l t dox a on ower ep a e car oxy c ac ‐mo e e ec ro escarboon (left) and gold (potentials [4]vs. Ag/AgCl reference electrode.COOHand sweep rate: 50 mV s‐1) A B.COO‐NH +COOH 3The CVs showed good behaviour for IDE of pyrolysed carbon(ΔE 150 V) d ld (ΔE 90 V) H f th b IDEp= m an go p= m . owever, or e car onCOO‐the thin and long leads of the electrode design caused increasedCOO NH +resistance resulting in higher ΔEp. ‐ 3OHA. CVs of 5 mM dopamine in PBS (pH7) OHCARBON GOLD on pyrolysed carbon: carboxylic acid‐       modified (blue) and plane (red) (theNyquist plots for          d / / )carbon and gold 2n CV is shown: potentials vs. Ag AgCl RE and sweep rate: 50 mV s .IDE i 1 M B. The proposed orientation of dopamine at carboxylic modified carbon electrode.s n m                      ruthenium (II/III)hexaammine (RUT),10mM potassiumb l d h b f d d• Car on e ectro es ave etter per ormance in opamine etectionhexacyanoferratecompared to gold and since it is technologically possible to fabricate(II/III) (FERR), PBSpH7 Inset: zoom incarbon microelectrode arrays these are preferable for dopamine. ‐f h i d ,do t e mpe anceexocytosis etection.spectra for gold• Carboxylic acid modified carbon electrodes improve the dopamineIDE ‐.orientation leading to stabilization of the electrode which can be,f l f l f d luse u or ong‐term amperometric monitoring o opamine re easefrom differentiating neruronal stem cellsInterestingly the semi‐circle in the impedance spectrograms seems .,d d h b lk f l d b l hto epen on t e u property o pyro yse car on on y, not on t eReferencessolution composition suggesting an intrinsic electroactive behaviour [1] M. K. Zachek, A. Hermans, R. M. Wightman, G. S. McCarty. J. Electroanal. Chem. 614 (2008) 113.[2] C S é l A H i k L H D Skj ldi J E é El l i 20 (2008) 680,. p ge , . e s anen, . . . o ng, . mn us. ectroana ys s .[3] C Spégel A Heiskanen J Acklid A Wolff R Taboryski J Emnéus T Ruzgas Electroanalysis 19 (2007) 263of the material . , . , . , . , . , . , . . .[4] A. Dalmia, C.C. Liu, R.F. Savinell. J. Electroanal. Chem. 430 (1997) 205..

Electrochemical evaluation of dopamine detection on pyrolysed carbon and gold electrodes

http://orbit.dtu.dk/ws/files/83624692/prod11389012746102.Electrochemical_evaluation_of_dopamine_detection_on_pyrolysed_carbon_and_gold_electrodes.pdf

Electrochemical evaluation of dopamine detection on pyrolysed carbon and gold electrodes

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology