CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
阴离子交换层析处理对静注人免疫球蛋白ph4制品的影响
Authors
吴静娟
安文琪
+6 more
张学成
李光飞
梁雪爽
王承勖
罗坚
马小伟
Publication date
1 January 2019
Publisher
Abstract
目的分析在低温乙醇蛋白分离法基础上引入阴离子交换层析(anion exchange chromatography,AEX)纯化工艺处理,对静注人免疫球蛋白(pH 4)制品质量的影响。方法检测并对比分析低温乙醇蛋白分离法及联合AEX纯化工艺制备的静注人免疫球蛋白(pH 4)制品分子量大小分布、抗补体活性(anticomplement activity,ACA)、抗-HBs效价、白喉抗体效价、IgA含量、抗体Fc段生物学活性、IgG亚型分布、N-糖基化类型、蛋白质二级结构和三级结构的异同。结果在低温乙醇蛋白分离法基础上,引入AEX纯化工艺处理,可大幅度降低静注人免疫球蛋白(pH 4)制品中IgA含量;有利于去除制品中三级结构发生改变的蛋白质;未对制品其他质量指标产生显著影响。结论在低温乙醇蛋白分离法基础上,引入AEX纯化工艺可提高静注人免疫球蛋白(pH 4)制品质量
Similar works
Full text
Available Versions
Institutional Repository of Institute of Process Engineering, CAS (IPE-IR)
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.ipe.ac.cn:122111/38151
Last time updated on 05/12/2019