超细SiO_2颗粒的制备及其对α-淀粉酶的吸附固定化

Authors
Publication date
1 January 2015
Publisher

Abstract

采用TritonX-100反胶团体系法和Stober法分别制备粒径为50nm及1 μm的SiO_2颗粒,用二氯二甲基硅烷(DDS)对其进行表面疏水性修饰,用于α-淀粉酶的固定化.结果表明,经DDS修饰的SiO_2颗粒对α-淀粉酶的吸附能力明显提高,且有效提高了吸附稳定性,经6次洗涤后酶活仅损失30%; 1 μm SiO_2颗粒比50 nm SiO_2颗粒对α-淀粉酶的吸附量大,SiO_2颗粒载体固定的α-淀粉酶的活性为50 nm SiO_2>1 μm SiO_2>50 nm修饰后SiO_2>1 μm修饰后SiO_2

    Similar works

    Full text

    thumbnail-image

    Available Versions

    Institutional Repository of Institute of Process Engineering, CAS (IPE-IR）

    redirect
    oaioai:ir.ipe.ac.cn:122111/32464
    Last time updated on 05/12/2019