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金鱼尾鳍组织的体外培养及血清对其生长的影响
Authors
戚艺华
聂峰光
贺丽君
Publication date
1 January 2001
Publisher
Abstract
采用组织块培养法对金鱼不同组织进行培养,并研究了血清对细胞生长的影响。在26℃温度下,采用添加15%胎牛血清的199培养基成功地获得了金鱼尾鳍细胞系。以2×10^5个/ml的细胞密度接种时,细胞倍增时间为3.3d,金鱼尾鳍细胞培养系统中的最适血清浓度为15%
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Last time updated on 05/12/2019