Canali, Chiara

Dufva, Martin

Emnéus, Jenny

Heiskanen, Arto

Wolff, Anders

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 17, 2017Bioimpedance based Lab-on-a-Chip (LOC) systems for tissue engineeringCanali, Chiara; Heiskanen, Arto; Wolff, Anders; Dufva, Martin; Emnéus, JennyPublication date:2012Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Canali, C., Heiskanen, A., Wolff, A., Dufva, M., & Emnéus, J. (2012). Bioimpedance based Lab-on-a-Chip (LOC)systems for tissue engineering. Poster session presented at 8th Protein.DTU Workshop, Kgs. Lyngby, Denmark.1  Bioimpedance based Lab-on-a-Chip (LOC) systems for tissue engineering Chiara Canali1, Arto Heiskanen1, Anders Wolff1, Martin Dufva1, Jenny Emnéus1 1DTU Nanotech, Dept. of Micro- and Nanotechnology, Technical University of Denmark, Kgs. Lyngby, Denmark chca@nanotech.dtu.dk  Brief description of research area:  The goal of our research is to develop a new proof of concept Lab-on-a-chip (LOC) system with integrated bioimpedance detection for the purpose of real time monitoring of the growth and viability of a vascularized 3D bioartificial liver-on-a-chip system (BAL-on-a-Chip). This project will be executed in synergy with the newly approved EU project NanoBio4Trans, which forms a new research direction within Nanotech’s LOC strategic field. The ultimate goal of the NanoBio4Trans project is to develop, optimise and validate a highly vascularised in vivo-like BAL as an extracorporeal bioartificial liver (EBAL) ready to be perfused with human blood plasma, in order to be exploited in the medical technology of the 21th century.   What we know:  The use of human induced pluripotent stem cells (hiPSC) can be exploited as the starting material to enable the construction of personalized artificial organs from a patient’s own cells. These cells can be grown and directed to differentiate into in vivo-like BALs by employing scalable and perfusable hybrid three dimensional scaffolds. Integrated optical and electrical biosensing systems can be used to monitor the effects and changes that occur during tissue growth. This allows control and surveillance of the BAL formation, with envisaged feed-back control. To establish new challenging bioimpedance sensing strategies with high sensitivity, accuracy and resolution in a 3D cell culture system and eliminate the influence of the capacitive effects of the electrode interface a specially adapted four-point electrode system will be designed and evaluated. Both commercial needle electrodes and home-made microelectrodes will be exploited.  Intra- and extracellular optical nanosensors (phosphorescence and fluorescence based) will be also developed and applied for a multi-parametric imaging and bioanalysis of cells, tissues and organs (integrity of vasculature, viability, O2, pH, liver function, differentiation markers, etc) working in close collaboration with Luxcel Biosciences (luxcel.com).  The perfusable hybrid scaffold and sensing systems will be integrated into the BAL support system enabling  real time monitoring and control of the effects of various parameters during its growth. 

Bioimpedance based Lab-on-a-Chip (LOC) systems for tissue engineering

http://orbit.dtu.dk/ws/files/83738843/Bioimpedance%20based%20Lab-on-a-Chip%20(LOC)%20systems%20for%20tissue%20engineering.pdf

Bioimpedance based Lab-on-a-Chip (LOC) systems for tissue engineering

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology