Al Atraktchi, Fatima Al-Zahraa

Bakmand, Tanya

Rømer Sørensen, Ane

Svendsen, Winnie Edith

The brain is the center of the nervous system, where serious neurodegenerative diseases such as Parkinson’s, Alzheimer’s and Huntington’s are products of functional loss in the neural cells (1). Typical techniques used to investigate these diseases lack precise control of the cellular surroundings, in addition to isolating the neural tissue from nutrient delivery and to creating unwanted gradients (2). This means that typical techniques used to investigate neurodegenerative diseases cannot mimic in vivo conditions, as closely as desired. We have developed a novel microfluidic system for culturing PC12 cells, neuronal cells, astrocytes cultures and brain slices. The microfluidic system provides efficient nutrient delivery, waste removal, access to oxygen, fine control over the neurochemical environment and access to modern microscopy. Additionally, the setup consists of an in vitro culturing and electrochemical sensor system that enables real time detection of metabolites, e.g. dopamine from cell cultures and brain slices. In summary we present results on culturing of brain slices and cells in the microfluidic system as well as on the incorporation of an electrochemical sensor system for characterization of exocytotic dopamine release from neural and brain cultures

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 17, 2017Culturing of PC12 Cells, Neuronal Cells, Astrocytes Cultures and Brain Slices in anOpen Microfluidic SystemAl Atraktchi, Fatima Al-Zahraa; Bakmand, Tanya; Rømer Sørensen, Ane; Svendsen, Winnie EdithPublication date:2013Link back to DTU OrbitCitation (APA):Al Atraktchi, F. A-Z., Bakmand, T., Rømer Sørensen, A., & Svendsen, W. E. (2013). Culturing of PC12 Cells,Neuronal Cells, Astrocytes Cultures and Brain Slices in an Open Microfluidic System. Poster session presentedat The Gordon Research Conference on the Physics and Chemistry of Microfluidics, Lucca, Italy.Culturing of PC12 Cells, Neuronal Cells, Astrocytes Cultures and Brain Slices in an Open Microfluidic System  Fatima AlZahra’a Alatraktchi, Tanya Bakmand, Ane Rømer Sørensen, Winnie E. Svendsen. Poster presentation at the Gordon Research Conference, Microfluidics, Physics and Chemistry, Tuscany, 2013.  The brain is the center of the nervous system, where serious neurodegenerative diseases such as Parkinson’s, Alzheimer’s and Huntington’s are products of functional loss in the neural cells (1).  Typical techniques used to investigate these diseases lack precise control of the cellular surroundings, in addition to isolating the neural tissue from nutrient delivery and to creating unwanted gradients (2).  This means that typical techniques used to investigate neurodegenerative diseases cannot mimic in vivo conditions, as closely as desired. We have developed a novel microfluidic system for culturing PC12 cells, neuronal cells, astrocytes cultures and brain slices. The microfluidic system provides efficient nutrient delivery, waste removal, access to oxygen, fine control over the neurochemical environment and access to modern microscopy. Additionally, the setup consists of an in vitro culturing and electrochemical sensor system that enables real time detection of metabolites, e.g. dopamine from cell cultures and brain slices. In summary we present results on culturing of brain slices and cells in the microfluidic system as well as on the incorporation of an electrochemical sensor system for characterization of exocytotic dopamine release from neural and brain cultures.  References: (1) D. Johnston, S. Wu, Foundations of Cellular Neurophysiology, Massachusetts Institute of Technology (1995) (2) Y. Huang, J. Williams, S. Johnson, Brain slice on a chip: opportunities and challenges of applying microfluidic technology to intact tissues, The Royal Society of Chemistry (2012) 

Culturing of PC12 Cells, Neuronal Cells, Astrocytes Cultures and Brain Slices in an Open Microfluidic System

http://orbit.dtu.dk/ws/files/83013733/prod11389169323876.Abstract_Tuscany_GRC_2013.pdf

Culturing of PC12 Cells, Neuronal Cells, Astrocytes Cultures and Brain Slices in an Open Microfluidic System

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology