Pilling and fuzz, which were not a problem at all in previous years (especially for cotton fabrics), have become a major problem recently. Fibre type, the yarn spinning system, fabric type, and finishing process play an important role in the pilling properties of fabrics. In this study, single jersey fabrics knitted with 100% combed, carded and open-end cotton yarns were used. An enzymatic process for fuzz reduction was applied at different stages, and the effect of this on pilling, strength, weight loss and the colour differences of the fabric were tested. Previous studies on this subject were carried out with small-scale samples, and therefore do not reflect the facts exactly. For this reason, all the phases of this study were specifically carried out under common working conditions like those prevailing in industry