Yam mosaic virus (YMV) is one of the most economically important virus infecting yams.
Immunoassays are routinely used for laboratory diagnosis of YMV and for certification of planting
materials. However, YMV antibodies, the key reagents, needed for these immunoassays are not readily
available. We describe in this paper, the production of YMV monoclonal antibodies for the detection of
YMV. The monoclonal antibody was produced by immunizing six weeks old BALB/c mice with YMV
hybridoma cells and tapping soft peritoneal tumor tissues for antibody. Antibody titre was determined
by triple antibody sandwich-enzyme-linked immunosorbent assay (TAS-ELISA) using YMV infected yam
leaves and non-infected tissue culture yam leaves. The antibody produced had a titre of 1:1,310,720 and
an optimal TAS-ELISA detection dilution of 1:80,000. This high-titre YMV monoclonal antibody is useful
for monitoring and certification purposes