'Japanese Association of Inorganic Phosphorus Chemistry'
Abstract
Not AvailableA total of fifty Bt isolates collected from various sources were used for screening for the presence of nematicidal crystal protein genes. Cry5 &Cry55 specific screening primers pairs were used for PCR screening along with reference strain which is positive for both cry5&Cry55 genes. The crystal morphology study revealed that majority of crystal proteins were amorphous and bipyramidal in nature, SDS-PAGE analysis of the crude protein mixture shown a prominent crystal protein band. In the PCR analysis, 13 isolates were found positive with cry5 specific primers and 23 isolates were found positive with cry 55 specific screening primers. Further five PCR fragments were sequenced, two PCR products were found identical to cry5B and three were found identical to cry55a genes with 99% similarity.Not Availabl
