Not AvailableCoconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant
cultivar Chowghat Green Dwarf (CGD) by using primers designed based on conserved motifs of the
NBS-LRR domain of the date palm. The amplifi ed sequences were cloned, sequenced and characterized. The
coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of
the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction
analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease
resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease
resistant traits in coconut.Not Availabl
