Not AvailableAn attempt was made to establish highly competent embryogenic cell suspension culture in coconut, a species
recalcitrant to in vitro culture. Embryogenic calli were initiated from shoot meristem explants of coconut. Y3
medium supplemented with 2.4-D (4.5 μM) and glutamine (34.2 μM) was found to be the best medium to initiate
cell suspension. Growth evaluation was done by packed cell volume (PCV) and it was found that maximum growth
volume of 9.9% was reached at 200 days of culture initiation. About 52% of viable cells were detected through
fluorescent microscopy. Cell aggregation was noticed in Y3 medium supplemented with glutamine (34.2 μM) ,
malt extract (100mg/l), biotin (40.9 μM) and kinetin (9.3 μM), but further progress could not be achieved. It was
also observed that embryogenic calli were not of a friable type, but were associated with densely aggregated
cells. Because of its hard nature, we were unsuccessful to obtain high quality cell suspension.Not Availabl
