Not AvailablePotato mop-top virus (PMTV) is the type species of genus Pomovirus, family
Virgaviridae. The occurrence of PMTV was reported recently in the Pacific
Northwestern USA. PMTV genome is characterized by three linear positive
sense ssRNA molecules. As part of a study to better understand the virus
biology and molecular biology, the genome of a WA isolate was characterized
and we report the complete nucleotide (nt) sequence of the genomic
components RNA-2, and RNA-3. RNA2 was 3134nt in length with an ORF
between nt positions 314-844 that codes for the coat protein and the same ORF as a read-through extends further to nt position 2791 coding for the CPread
through protein. RNA 3 was 2964nt in length and potentially encodes
four ORFs – three of them forming the triple gene bock (TGB) that was
shown to be involved in cell to cell movement. RNA2 also contains a 206 nt
ORF present toward the 3’ end that encodes a cysteine-rich 8K protein. RFLP
analysis of RNA2 and RNA 3 of the WA isolate revealed the combination of
RNA2-II and RNA3-B types, the combination that is prevalent in Europe and
elsewhere. The sequenced regions had 99% sequence identity with the
corresponding RNA components reported from Europe. The high degree of
sequence identity could be useful in developing virus detection tools and for
designing RNAi-based virus management strategies.Not Availabl
