Not AvailableThe present study was explained about a direct
and indirect regeneration protocol for an important wild
medicinal plant Dioscorea deltoidea, a rich source of
saponins and diosgenin. It was found that source of
explant, basal medium composition, concentration of plant
growth regulator and the photoperiod directly control
morphogenetic responses. Out of all explants tested, only
nodal segments performed better on the experimental
growth media. Nodal segments initiated direct organogenesis
when cultured on MS and RT (revised tobacco)
medium supplemented with BAP and NAA or IBA.
Growth medium supplemented with BAP (1.5 mg/l) and
IBA (0.5 mg/l) induced maximum regeneration. Indirect
roots regeneration was achieved on RT medium supplemented
with IAA 1.0 mg/l and RT medium supplemented
with BAP 1.0 mg/l and NAA 0.5 mg/l induced shoot
regeneration through callus formed on MS medium containing
2, 4-D (2.0 mg/l).Not Availabl
