Not AvailableBrucellosis caused by Brucella species is readily transmissible to humans, causing acute
febrile illness and undulant fever which may progress to a more chronic form and can also
produce serious complications affecting the musculoskeletal, cardiovascular, and central
nervous systems. A veterinary livestock inspector presented to the institute with symptoms
of intermittent fever, pain involving muscles and joints, loss of weight, anxiety and weakness
for about three months has been investigated. The isolation, serological tests and PCR were
performed for diagnosis of brucellosis. Based on history of constant professional association
with animals, characteristic symptoms, hematological and biochemical, multiple serological
and PCR assay results, the patient was diagnosed as brucellosis. Detection of Brucella abortus
directly in the clinical samples by gel based PCRs were highly useful for diagnosis and
monitoring of treatment. This diagnostic protocol will facilitate in a simple way to map major
Brucella species infecting humans in a geographical region.Not Availabl
