Not AvailableParticle bombardment/biolistic delivery is a very popular method of genetic transformation of diverse
targets including cells and intact tissues. Delivery of DNA through particle bombardment is genotype and
species independent, nevertheless, an ef fi cient protocol for large-scale generation of transgenic plants
through embryogenic tissues with a high ( ³ 80%) shoot regeneration ef fi ciency is a prerequisite. Young
embryogenic tissues or multiple shoot buds in early stages of induction are the most suited target tissues
for recovery of transgenic plants. We describe the protocol for delivery of foreign genes using particle
delivery system (Biorad gene gun, PDS-1000/He) in to the meristematic tissues of embryonic axes derived
from mature seeds of castor. With the optimized physical and biological parameters, putative transformants
were obtained at a frequency of 1.4% through particle gun bombardment of castor embryo axes. Also,
transformation of embryogenic calli of sorghum using particle in fl ow gun (PIG) is described.Not Availabl
