Not AvailableTwenty-four diverse genotypes of sorghum were
evaluated for response to callus induction and plant regeneration
with two media viz., MS and NBKNB using shoot
tips as the start-material to identify a model genotype. None
of the genotypes tested showed promising results. Therefore,
alternative methods of in vitro pathways using shoot
meristem isolated from shoot tips were explored. Shoot
apical meristems were isolated and were induced to multiple
shoots or multiple shoot buds pathway by manipulation
of thidiazuron (TDZ), 6-benzyl adenine (BAP) and 2, 4-
dichlorophenoxy acetic acid (2, 4-D). Choice of the pathway
whether large-scale multiplication of shoots or production
of target tissues for transformation can be exercised
based on the needs and applications. A simple procedure,
for large scale handling of shoot tips is described in detail.
Electron microscopic studies revealed that meristems isolated
from 7-day-old seedlings are superior because of possessing
greater number of transformation competent cells.Not Availabl
