Zinc oxide nanoparticles were synthesized in order to get biologically active additive for correction of zinc-deficiency states. Nanosized zinc oxide was derived by through condensation method from zinc acetate and lithium hydroxide dissolved in dehydrated alcohol. Resulted particles were examined through method of small-angle X-ray scattering. Measurement results confirm presence of nanoparticles with average size about 2 nm.
When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3365

Borsch, N.A.

Budko, E.V.

Gorbacheva, L.A.

Khabarov, A.A.

Larin, S.L.

Orlov, E.Y.

SocioEconomic Challenges Journal (Sumy State University)

JOURNAL OF NANO- AND ELECTRONIC PHYSICS ЖУРНАЛ НАНО- ТА ЕЛЕКТРОННОЇ ФІЗИКИ 
Vol. 5 No 4, 04032(2pp) (2013) Том 5 № 4, 04032(2cc) (2013) 
 
 
The article was reported at the International Conference «Physics and Technology of Nanomaterials and Structures», Kursk, 21-22 November, 2013 
2077-6772/2013/5(4)04032(2) 04032-1  2013 Sumy State University 
Short Communication 
 
Synthesis and Identification of Zinc Oxide Nanoparticles as Precursor for Getting Zinc-
Based Biologically Active Additives 
 
S.L. Larin1, E.Y. Orlov2, N.A. Borsch2, L.A. Gorbacheva2, E.V. Budko1, A.A. Khabarov1 
 
1 Kursk State Medical University, 3, Karla-Marksa Srt., 305041 Kursk, Russian Federation 
2 Southwest State University, 94, 50 let Octyabrya, 305040 Kursk, Russian Federation 
 
(Received 01 October 2013; revised manuscript received 05 November 2013; published online 10 December 2013) 
 
Zinc oxide nanoparticles were synthesized in order to get biologically active additive for correction of 
zinc-deficiency states. Nanosized zinc oxide was derived by through condensation method from zinc acetate 
and lithium hydroxide dissolved in dehydrated alcohol. Resulted particles were examined through method 
of small-angle X-ray scattering. Measurement results confirm presence of nanoparticles with average size 
about 2 nm. 
 
Keywords: Hypozincosis, Precursor, Biologically active additive, Zinc oxide nanoparticles, Small-angle  
X-ray scattering. 
 
 PACS number: 81.16.Be 
 
 
A certain microelement balance must be supported 
within the organism for normal behavior of vital pro-
cesses. Zinc may be marked among dozens of other es-
sential microelements. Zn2 + is included in the structure 
of active centers of more than 100 metalloenzymes, 
provides healthy state of thymus and immunity, affects 
growth / aging of neurons and neurotransmitter syn-
thesis, plays a key role in generation of normal sexual 
function of men [1]. 
Light or moderate deficiency of zinc comes out in 
immunity decrease and liability to contagious and non-
contagious diseases: ischemic heart disease, atheroscle-
rosis, diabetes mellitus and some others. Serious zinc 
deficiency of pregnant women may cause congenital mal-
formations [1]. More than 2 billion people around the 
world do not get enough zinc from their ration. Zink de-
ficiency is an important health protection issue in ad-
vanced countries. According to figures from the Interna-
tional Zinc Association, zinc-deficiency states are regis-
tered almost everywhere. Moderate hypozincosis is 
widespread in Europe, Brazil; serious zinc deficiencies 
are registered in India, South Africa. Zink deficiency 
cases occur among population of Russia as well [1, 2]. 
Various zinc-containing biologically active additives 
may be used for correction of moderate zinc-deficiency 
states. Different preparations for hypozincosis correc-
tion are represented in the pharmaceutical market. 
Despite their widespread distribution, such prepara-
tions can not provide optimal bioavailability of zinc, 
concentrations of Zn2 + are extremely high. 
All traditional pharmaceutical forms have a set of 
fundamental defects: excessive medicinal substance 
consumption, undirected operation of medical sub-
stance, insufficient biocompatibility and bioavailability. 
The march of modern science allows creating brand 
new pharmaceutical forms without defects listed above. 
Their improved properties can be attributed to size 
reduction. Nanosized medicinal particles have series of 
advantages: increased bioavailability, provision of op-
timal dosage, minimization of side effects, etc. [1]. 
 
The purpose of this research was to derive and 
identify nanoparticles of zinc oxide that are proposed to 
be used as the general component of preparation for 
zinc-deficiency correction. 
Synthesis of nanoparticles was carried out through 
condensation method in the environment of dehydrated 
ethanol. Rectified ethanol with 99.6 % concentration 
was dehydrated through boiling with calcium metal 
during 3 hours and further distillation. 
For derivation of nanoparticles, 0.15 g of lithium 
hydroxide and 0.55 g of zinc acetate were dissolved in 
50 ml of dehydrated alcohol during 15 minutes at con-
tinuous stirring with magnetic mixer. Zinc acetate was 
dissolved at the boiling temperature. After dissolution, 
lithium hydroxide and zinc acetate solutions were 
cooled to 0 С at continuous stirring. After that, zinc 
acetate solution was added to another solution at a 
speed of several drops per second. The solution was 
heated to the room temperature; after that, 60 ml of 
deionized water were added to it. The resulted suspen-
sion was centrifuged. The resulted precipitation was 
dried during 12 hours under vacuum at 90 С and re-
dried for 3 hours in desiccator at 120 С. 
 
 
 
Fig. 1 – Distribution curve by dimensions for zinc oxide nano-
particles according to small-angle X-ray scattering data 
r, nm 
 S.L. LARIN, E.Y. ORLOV, N.A. BORSCH, ET. AL J. NANO- ELECTRON. PHYS. 5, 04032 (2013) 
 
 
04032-2 
The method of small-angle X-ray scattering was 
used for identification of zinc oxide nanoparticles. 
Measurement was carried out by means of small-angle 
X-ray scattering installation SAXSess mc2 from Anton 
Paar, Austria. During measurement process, vacuum 
with pressure of about 0.3 mbar is created in the sam-
ple chamber. The installation was set to X-ray wave-
length () of 0.1542 nm. Functions of volume distribu-
tion of nanoparticles by DV(r) dimensions were calcu-
lated on the computer according to small-angle scatter-
ing intensity curves. Computer processing of experi-
ment data was carried out with the use of GNOM soft-
ware. As shown in the figure below, the majority (near-
ly 80 %) of particles contained in the gel is represented 
in size of about 2 nm. Larger aggregates are also met – 
12 nm, 22 nm and 38 nm. 
Therefore, zinc oxide nanoparticles with size of 
about 2 nm were successfully derived as the result of 
the experiment. Such particles may be used as active 
ingredient in a preparation for correction of zinc defi-
ciency. 
 
 
REFERENCES 
 
1. A.V Skalny, I.A Rudakov, Bioelements in Medicine  
(Moscow: Mir: 2004). 
2. H. Michael, J. Nutrition 130, 1344S (2000). 


English

Synthesis and Identification of Zinc Oxide Nanoparticles as Precursor for Getting Zinc-Based Biologically Active Additives

Electronic Sumy State University Institutional Repository

JOURNAL OF NANO- AND ELECTRONIC PHYSICS ЖУРНАЛ НАНО- ТА ЕЛЕКТРОННОЇ ФІЗИКИ Vol. 5 No 4, 04032(2pp) (2013) Том 5 № 4, 04032(2cc) (2013)   The article was reported at the International Conference «Physics and Technology of Nanomaterials and Structures», Kursk, 21-22 November, 2013 2077-6772/2013/5(4)04032(2) 04032-1  2013 Sumy State University Short Communication  Synthesis and Identification of Zinc Oxide Nanoparticles as Precursor for Getting Zinc-Based Biologically Active Additives  S.L. Larin1, E.Y. Orlov2, N.A. Borsch2, L.A. Gorbacheva2, E.V. Budko1, A.A. Khabarov1  1 Kursk State Medical University, 3, Karla-Marksa Srt., 305041 Kursk, Russian Federation 2 Southwest State University, 94, 50 let Octyabrya, 305040 Kursk, Russian Federation  (Received 01 October 2013; revised manuscript received 05 November 2013; published online 10 December 2013)  Zinc oxide nanoparticles were synthesized in order to get biologically active additive for correction of zinc-deficiency states. Nanosized zinc oxide was derived by through condensation method from zinc acetate and lithium hydroxide dissolved in dehydrated alcohol. Resulted particles were examined through method of small-angle X-ray scattering. Measurement results confirm presence of nanoparticles with average size about 2 nm.  Keywords: Hypozincosis, Precursor, Biologically active additive, Zinc oxide nanoparticles, Small-angle  X-ray scattering.   PACS number: 81.16.Be   A certain microelement balance must be supported within the organism for normal behavior of vital pro-cesses. Zinc may be marked among dozens of other es-sential microelements. Zn2 + is included in the structure of active centers of more than 100 metalloenzymes, provides healthy state of thymus and immunity, affects growth / aging of neurons and neurotransmitter syn-thesis, plays a key role in generation of normal sexual function of men [1]. Light or moderate deficiency of zinc comes out in immunity decrease and liability to contagious and non-contagious diseases: ischemic heart disease, atheroscle-rosis, diabetes mellitus and some others. Serious zinc deficiency of pregnant women may cause congenital mal-formations [1]. More than 2 billion people around the world do not get enough zinc from their ration. Zink de-ficiency is an important health protection issue in ad-vanced countries. According to figures from the Interna-tional Zinc Association, zinc-deficiency states are regis-tered almost everywhere. Moderate hypozincosis is widespread in Europe, Brazil; serious zinc deficiencies are registered in India, South Africa. Zink deficiency cases occur among population of Russia as well [1, 2]. Various zinc-containing biologically active additives may be used for correction of moderate zinc-deficiency states. Different preparations for hypozincosis correc-tion are represented in the pharmaceutical market. Despite their widespread distribution, such prepara-tions can not provide optimal bioavailability of zinc, concentrations of Zn2 + are extremely high. All traditional pharmaceutical forms have a set of fundamental defects: excessive medicinal substance consumption, undirected operation of medical sub-stance, insufficient biocompatibility and bioavailability. The march of modern science allows creating brand new pharmaceutical forms without defects listed above. Their improved properties can be attributed to size reduction. Nanosized medicinal particles have series of advantages: increased bioavailability, provision of op-timal dosage, minimization of side effects, etc. [1].  The purpose of this research was to derive and identify nanoparticles of zinc oxide that are proposed to be used as the general component of preparation for zinc-deficiency correction. Synthesis of nanoparticles was carried out through condensation method in the environment of dehydrated ethanol. Rectified ethanol with 99.6 % concentration was dehydrated through boiling with calcium metal during 3 hours and further distillation. For derivation of nanoparticles, 0.15 g of lithium hydroxide and 0.55 g of zinc acetate were dissolved in 50 ml of dehydrated alcohol during 15 minutes at con-tinuous stirring with magnetic mixer. Zinc acetate was dissolved at the boiling temperature. After dissolution, lithium hydroxide and zinc acetate solutions were cooled to 0 С at continuous stirring. After that, zinc acetate solution was added to another solution at a speed of several drops per second. The solution was heated to the room temperature; after that, 60 ml of deionized water were added to it. The resulted suspen-sion was centrifuged. The resulted precipitation was dried during 12 hours under vacuum at 90 С and re-dried for 3 hours in desiccator at 120 С.    Fig. 1 – Distribution curve by dimensions for zinc oxide nano-particles according to small-angle X-ray scattering data r, nm  S.L. LARIN, E.Y. ORLOV, N.A. BORSCH, ET. AL J. NANO- ELECTRON. PHYS. 5, 04032 (2013)   04032-2 The method of small-angle X-ray scattering was used for identification of zinc oxide nanoparticles. Measurement was carried out by means of small-angle X-ray scattering installation SAXSess mc2 from Anton Paar, Austria. During measurement process, vacuum with pressure of about 0.3 mbar is created in the sam-ple chamber. The installation was set to X-ray wave-length () of 0.1542 nm. Functions of volume distribu-tion of nanoparticles by DV(r) dimensions were calcu-lated on the computer according to small-angle scatter-ing intensity curves. Computer processing of experi-ment data was carried out with the use of GNOM soft-ware. As shown in the figure below, the majority (near-ly 80 %) of particles contained in the gel is represented in size of about 2 nm. Larger aggregates are also met – 12 nm, 22 nm and 38 nm. Therefore, zinc oxide nanoparticles with size of about 2 nm were successfully derived as the result of the experiment. Such particles may be used as active ingredient in a preparation for correction of zinc defi-ciency.   REFERENCES  1. A.V Skalny, I.A Rudakov, Bioelements in Medicine  (Moscow: Mir: 2004). 2. H. Michael, J. Nutrition 130, 1344S (2000). 

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Synthesis and Identification of Zinc Oxide Nanoparticles as Precursor for Getting Zinc-Based Biologically Active Additives

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