Agier

Alessandro P.S. de Moura

Alvino

Besnard

Bouton

Breier

Carolin A. Müller

Celniker

Chang

Conrad A. Nieduszynski

Cosgrove

Crampton

de Moura

De Piccoli

Di Rienzi

Eaton

Feng

Friedman

Gilbert

Hoggard

Huang

Katsuhiko Shirahige

Kearsey

Knott

Koren

Liachko

Lian

Makiko Komata

Martin J. Blythe

McGuffee

Mechali

Mesner

Michelle Hawkins

Muller

Nakato

Natsume

Newman

Nieduszynski

Palzkill

Raghuraman

Ray Wilson

Renata Retkute

Retkute

Rhind

Rudolph

Ryuichiro Nakato

Sekedat

Sharma

Shirahige

Siow

Sunir Malla

Theis

Vujcic

Yabuki

Yamashita

Yoshikawa

Nucleic Acids Research

English

PubMed

Eukaryotic genomes are replicated from multiple DNA replication origins. We present complementary deep sequencing approaches to measure origin location and activity in Saccharomyces cerevisiae. Measuring the increase in DNA copy number during a synchronous S-phase allowed the precise determination of genome replication. To map origin locations, replication forks were stalled close to their initiation sites; therefore, copy number enrichment was limited to origins. Replication timing profiles were generated from asynchronous cultures using fluorescence-activated cell sorting. Applying this technique we show that the replication profiles of haploid and diploid cells are indistinguishable, indicating that both cell types use the same cohort of origins with the same activities. Finally, increasing sequencing depth allowed the direct measure of replication dynamics from an exponentially growing culture. This is the first time this approach, called marker frequency analysis, has been successfully applied to a eukaryote. These data provide a high-resolution resource and methodological framework for studying genome biology

Müller, Carolin A.

Hawkins, Michelle

Retkute, Renata

Malla, Sunir

Wilson, Ray

Blythe, Martin J.

Nakato, Ryuichiro

Komata, Makiko

Shirahige, Katsuhiko

de Moura, Alessandro P.S.

Nieduszynski, Conrad A.

Name not available

The dynamics of genome replication using deep sequencing

M�ller, Carolin A.

Repository@Nottingham

Crossref

Nottingham eTheses

Müller, Carolin A

Blythe, Martin J

de Moura, Alessandro P S

Nieduszynski, Conrad A

The Open University

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de Moura, Alessandro P. S.

University of East Anglia digital repository

Nottingham ePrints

http://eprints.nottingham.ac.uk/2524/1/Conrad_Nieduszynski--dynamics_of_genome.pdf

The dynamics of genome replication using deep sequencing

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Name not available

Name not available

Repository@Nottingham

Crossref

Nottingham eTheses

The Open University

University of East Anglia digital repository

Nottingham ePrints