A Corrigan

A Reyes

AJ Scupham

BB Oakley

BY Liu

C De Filippo

C Quast

C Zhang

DH Parks

E Jami

F Vasaï

H Chung

H Mikkelsen

I Sekirov

J Vulevic

JR Cole

K Borin

KM Singh

L Jin

L Zhao

LW He

M Sekelja

MAM Shaufi

MJ Sergeant

N Segata

P Borst

PD Schloss

PT Lan

S Kumar

TZ Desantis

X Han

YH Luo

Z Bo

ZY Wang

Scientific Reports

AbstractGeese are extremely adept in utilizing plant-derived roughage within their diet. However, the intestinal microbiome of geese remains limited, especially the dietary effect on microbial diversity. Cassava foliage was widely used in animal feed, but little information is available for geese. In this study, the geese were fed with control diet (CK), experimental diet supplemented with 5% cassava foliage (CF5) or 10% (CF10) for 42 days, respectively. The cecal samples were collected after animals were killed. High-throughput sequencing technology was used to investigate the microbial diversity in the caecum of geese with different dietary supplements. Taxonomic analysis indicated that the predominant phyla were distinct with different dietary treatments. The phyla Firmicutes (51.4%), Bacteroidetes (29.55%) and Proteobacteria (7.90%) were dominant in the CK group, but Bacteroidetes (65.19% and 67.29%,) Firmicutes (18.01% and 17.39%), Proteobacteria (8.72% and 10.18%), Synergistete (2.51% and 1.76%) and Spirochaetes (2.60% and 1.46%) were dominant in CF5 and CF10 groups. The abundance of Firmicutes was negatively correlated with the supplementation of cassava foliage. However, the abundance of Bacteroidetes and Proteobacteria were positively correlated with the supplementation of cassava foliage. Our study also revealed that the microbial communities were significantly different at genus levels. Genes related to nutrient and energy metabolism, immunity and signal transduction pathways were primarily enriched by the microbiome.</jats:p

Mao Li

Hanlin Zhou

Xiangyu Pan

Tieshan Xu

Zhenwen Zhang

Xuejuan Zi

Yu Jiang

Crossref

Cassava foliage affects the microbial diversity of Chinese indigenous geese caecum using 16S rRNA sequencing

Cassava foliage affects the microbial diversity of Chinese indigenous geese caecum using 16S rRNA sequencing

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