Bumsted RM

Coles NW

de Jonge BL

Jacob GS

Joseph A. H. Romaniuk

Lynette Cegelski

Navarre WW

Pan Y

Siewert G

Strominger JL

Tipper DJ

Yocum RR


            The ability to characterize bacterial cell-wall composition and structure is crucial to understanding the function of the bacterial cell wall, determining drug modes of action and developing new-generation therapeutics. Solid-state NMR has emerged as a powerful tool to quantify chemical composition and to map cell-wall architecture in bacteria and plants, even in the context of unperturbed intact whole cells. In this review, we discuss solid-state NMR approaches to define peptidoglycan composition and to characterize the modes of action of old and new antibiotics, focusing on examples in
            Staphylococcus aureus
            . We provide perspectives regarding the selected NMR strategies as we describe the exciting and still-developing cell-wall and whole-cell NMR toolkit. We also discuss specific discoveries regarding the modes of action of vancomycin analogues, including oritavancin, and briefly address the reconsideration of the killing action of β-lactam antibiotics. In such chemical genetics approaches, there is still much to be learned from perturbations enacted by cell-wall assembly inhibitors, and solid-state NMR approaches are poised to address questions of cell-wall composition and assembly in
            S. aureus
            and other organisms.
          </jats:p

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Philosophical Transactions of the Royal Society B Biological Sciences

Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR

Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR

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