AS Haka

B Madajewski

CA Metildi

D Holt

EC Rossi

EH Shin

F Aydogan

GA Wagnieres

H Eagle

J Benard

J Predina

JL Foo

JR Van der Vorst

JX Jiang

KY Lin

M Hutteman

M Krieg

M Zellweger

S Tobis

SL Gibbs-Strauss

SL Troyan

T Ishizawa

TJ Collins

V Ntziachristos

Y Liu

Y Matsumara

Scientific Reports

AbstractIntraoperative optical cancer imaging is an emerging technology in which surgeons employ fluorophores to visualize tumors, identify tumor-positive margins and lymph nodes containing metastases. This study compares instrumentation to measure tumor fluorescence. Three imaging systems (Spectropen, Glomax, Flocam) measured and quantified fluorescent signal-to-background ratios (SBR) in vitro, murine xenografts, tissue phantoms and clinically. Evaluation criteria included the detection of small changes in fluorescence, sensitivity of signal detection at increasing depths and practicality of use. In vitro, spectroscopy was superior in detecting incremental differences in fluorescence than luminescence and digital imaging (Ln[SBR] = 6.8 ± 0.6, 2.4 ± 0.3, 2.6 ± 0.1, p = 0.0001). In fluorescent tumor cells, digital imaging measured higher SBRs than luminescence (6.1 ± 0.2 vs. 4.3 ± 0.4, p = 0.001). Spectroscopy was more sensitive than luminometry and digital imaging in identifying murine tumor fluorescence (SBR = 41.7 ± 11.5, 5.1 ± 1.8, 4.1 ± 0.9, p = 0.0001) and more sensitive than digital imaging at detecting fluorescence at increasing depths (SBR = 7.0 ± 3.4 vs. 2.4 ± 0.5, p = 0.03). Lastly, digital imaging was the most practical and least time-consuming. All methods detected incremental differences in fluorescence. Spectroscopy was the most sensitive for small changes in fluorescence. Digital imaging was the most practical considering its wide field of view, background noise filtering capability and sensitivity to increasing depth.</jats:p

Ryan P. Judy

Jane J. Keating

Elizabeth M. DeJesus

Jack X. Jiang

Olugbenga T. Okusanya

Shuming Nie

David E. Holt

Sean P. Arlauckas

Phillip S. Low

E. James Delikatny

Sunil Singhal

Crossref

Quantification of tumor fluorescence during intraoperative optical cancer imaging

Quantification of tumor fluorescence during intraoperative optical cancer imaging

Abstract

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