A Mikstacki

AI Svirnovski

AL Mourão

CE Petersen

D Booven Van

D Stewart

E Martignoni

EL Woodahl

F Kansaku

H Jia

I Hernan

I Loryan

JG Restrepo

L Coulbault

L Pasin

M Schywalsky

M Sousa Dias de

MS Khan

NP Franks

O Mastrogianni

O Zakerska

P Korprasertthaworn

R Landau

RB Raftogianis

RD Traver

SJ Wolf

U Kragh-Hansen

UM Zanger

Y Zhang

Scientific Reports

AbstractThe individual response of patients to propofol results from the influence of genetic factors. However, the state of knowledge in this matter still remains insufficient. The aim of our study was to determine genetic predictors of variable pharmacokinetics and pharmacodynamics of propofol within selected 9 genes coding for propofol biotransformation enzymes, receptors and transporters. Our studies are the first extensive pharmaocgenetics research of propofol using high throughput sequencing technology. After the design and optimization of long range PCR-based next-generation sequencing experiment, we screened promoter and coding sequences of all genes analyzed among 87 Polish patients undergoing general anaesthesia with propofol. Initially we found that two variants, c.516 G &gt; T in the CYP2B6 gene and c.2677 T &gt; G in the ABCB1 gene, significantly correlate with propofol’s metabolic profile, however after Bonferroni correction the P-values were not statistically significant. Our results suggest, that variants within the CYP2B6 and ABCB1 genes correlate stronger with propofol’s metabolic profile compared to other 7 genes. CYP2B6 and ABCB1 variants can play a potentially important role in response to this anaesthetic and they are promising object for further studies.</jats:p

Oliwia Zakerska-Banaszak

Marzena Skrzypczak-Zielinska

Barbara Tamowicz

Adam Mikstacki

Michal Walczak

Michal Prendecki

Jolanta Dorszewska

Agnieszka Pollak

Urszula Lechowicz

Monika Oldak

Kinga Huminska-Lisowska

Marta Molinska-Glura

Marlena Szalata

Ryszard Slomski

Crossref

Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia

Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia

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