Bibikova

Catherine A. Makarewich

Douglas M. Anderson

Eric N. Olson

Gulick

John McAnally

Kelli J. Carroll

Lorena Zentilin

Mauro Giacca

Ning Liu

Rhonda Bassel-Duby

Significance
          
            The recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)9 system has greatly simplified the process of genomic editing. However, it has remained difficult to induce mutations in postnatal animals due to delivery challenges of the CRISPR/Cas9 components. Here, we report the generation of a transgenic mouse line that expresses Cas9 exclusively in cardiomyocytes. By using Adeno-Associated Virus 9 to deliver single-guide RNA (sgRNA) this method can rapidly induce genomic insertions and deletions in the heart. As proof of concept, administration of sgRNA against the
            Myh6
            gene induced
            Myh6
            editing, resulting in cardiomyopathy and heart failure in the cardiac-specific Cas9 mouse. This transgenic mouse model offers a valuable tool for cardiovascular research, as a straightforward strategy to edit genes of interest in the heart.
          </jats:p

Crossref

Proceedings of the National Academy of Sciences

A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9

A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9

Abstract

Similar works

Full text

Available Versions

Crossref