AK Darius

B Hoffmann

D Li

DM Kolpashchikov

GA Boulet

H Mollasalehi

H Ravan

H Wu

J Compton

JT van Oirschot

M Deng

P Ning

P Travascio

S Chimeno Zoth

S Edwards

S Suradhat

T Kievits

V Moennig

W Zhou

X Zhao

XJ Zhang

Y Long

Z Zeng

Scientific Reports

AbstractThe split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Here, we successfully integrated colorimetric split G-quadruplex DNAzyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the RNA of Shimen and HCLV strains of Classic Swine Fever Virus (CSFV). CSFV is a RNA virus that causes a highly contagious disease in domestic pigs and wild boar. With this method, we were able to detect as little as 10 copies/ml of CSF viral RNA within 3 h in serum samples taken from the field. No interference was encountered in the amplification and detection of Classic Swine Fever Virus in the presence of non-target RNA or DNA. Moreover, Shimen and HCLV strains of Classic Swine Fever Virus could be easily differentiated using the NASBA-DNAzyme system. These findings indicate the NASBA-DNAzyme system is a rapid and practical technique for detecting and discriminating CSFV strains and may be applied to the detection of other RNA viruses.</jats:p

Xiaolu Lu

Xueyao Shi

Gege Wu

Tiantian Wu

Rui Qin

Yi Wang

Crossref

Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay

Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay

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