A Watthanasurorot

AF Williams

AN Zelensky

B Pees

CA Janeway

CM Adema

D Brites

D Schmucker

F Guillou

FL Watson

GW Litman

JS Kang

JV Ravetch

K Tamura

MM Bradford

NM Dheilly

P Jiravanichpaisal

PH Chou

PM Leonard

PS Gross

R Bettencourt

RB Dodd

S Wang

T Luo

U Holmskov

UK Laemmli

XJ Du

XW Wang

XW Zhang

Y Yamaguchi

YD Sun

Z Pancer

Scientific Reports

AbstractC-type lectins are important immune molecules that participate in host defense response. The present work reports a novel C-type lectin (PcLec3) from the red swamp crayfish Procambarus clarkii. Sequence analysis found that PcLec3 encodes a polypeptide with252 amino acid residues, which contains an immunoglobulin-like domain (IG) and a C-type lectin domain (CTLD) arranged in tandem. Tissue distribution analysis indicated that PcLec3 is enriched expressed in hemocytes and hepatopancreas cells, in which PcLec3 was up-regulated following bacterial challenge by Vibrio anguillarum. Function analysis using recombinant full-length PcLec3, IG and CTLD proteins revealed that these recombinant proteins had the capacity to bind carbohydrates and bacteria, while IG determined the cell binding activity. However, only full-length PcLec3 promotes the phagocytic activity of hemocytes and subsequent clearance of invasive bacteria. Taken together, these results manifest that PcLec3 acts as a hemocyte adhesion molecule to promote hemocyte phagocytosis against invasive V. anguillarum.</jats:p

Xiao-Wen Zhang

Yue Wang

Xian-Wei Wang

Lei Wang

Yi Mu

Jin-Xing Wang

Crossref

A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii

A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii

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