Boas, Ulrik

The invention relates to a method of producing a polysaccharide-carrier conjugate comprising coupling a polysaccharide to a carrier, said polysaccharide comprising at least one monosaccharide unit comprising a keto-carboxy group according to the formula -C(=O)COOR, where R is either hydrogen or C1-C6 alkyl or a ringclosed ketal or hemi-ketal corresponding thereto, said method comprising the steps of, i) providing a carrier comprising N-hydroxylamine- or N-alkoxyamine groups according to the formula -NHOR, wherein R is H or C1-C6 alkyl attached thereto, ii) reacting said N-hydroxylamine or N-alkoxyamine group of the carrier with a keto-carboxy group of said polysaccharide to form a covalent amide bond between the carrier and the polysaccharide. Another aspect of the present invention relates to a compound or solid surface obtained when performing the method of the present invention. A third aspect of the present invention relates to the use of the compound or solid surface in the preparation of an assay device for the detection of antibodies against gram negative bacteria

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Improved coupling of bacterial polysaccharides to macromolecules and solid supports

http://orbit.dtu.dk/ws/files/81310579/WO2013060335A1.pdf

Improved coupling of bacterial polysaccharides to macromolecules and solid supports

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