Feidenhans'l, Nikolaj Agentoft

Jensen, Thomas Glasdam

Kutter, Jörg Peter

Lafleur, Josiane P.

We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidicchip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlationbetween streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency andrefractive index of the cured polymer

Online Research Database In Technology

Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Feidenhans, Nikolaj A.

Kutter, Jörg P.

Copenhagen University Research Information System

Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

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Online Research Database In Technology

Copenhagen University Research Information System