A Nakao

D Thomas

E Wilhelm

F Kur

G Segall

H Kato

I Bauer

I Heron

J Kohmoto

J Yoshida

JE Stahl

K Waki

KJ Zehr

LP Perrault

MH Maathuis

ML Cobert

N Hatayama

N Murase

PK Linden

PS Macdonald

Q Wang

R Schmidt

S Yoshida

SR Jones

TJ Wheeler

TP Fitton

TR Türk

Y Kamoda

Y Kapanci

Y Li

Scientific Reports

AbstractWe recently succeeded in resuscitating an extracted rat heart following 24–48 hours of preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). This study aimed to examine the function of rat hearts transplanted after being preserved in the high-pressure CO and O2 gas mixture. The hearts of donor rats were preserved in a chamber filled with CO and O2 under high pressure for 24 h (CO24h) or 48 h at 4 °C. For the positive control (PC) group, hearts immediately extracted from donor rats were used for transplantation. The preserved hearts were transplanted into recipient rats by heterotopic cervical heart transplantation. CO toxicity does not affect the grafts or the recipients. Light microscopy and [18F]-fluorodeoxyglucose positron emission tomography revealed that there were no significant differences in the size of the myocardial infarction or apoptosis of myocardial cells in post-transplant hearts between the PC and CO24h groups. Furthermore, at 100 days after the transplantation, the heart rate, weight and histological staining of the post-transplanted hearts did not differ significantly between the PC and CO24h groups. These results indicate that the function of rat hearts is well preserved after 24 hours of high-pressure preservation in a CO and O2 gas mixture. Therefore, high-pressure preservation in a gas mixture can be a useful method for organ preservation.</jats:p

Naoyuki Hatayama

Masayuki Inubushi

Munekazu Naito

Shuichi Hirai

Yong-Nan Jin

Atsushi B. Tsuji

Kunihiro Seki

Masahiro Itoh

Tsuneo Saga

Xiao-Kang Li

Crossref

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen

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