A Boveris

A Grzelak

A Reynolds

B Halliwell

D Jacewicz

D Wyrzykowski

E Kita

E Schleicher

G Rothe

HJH Fenton

J Friedman

J Pranczk

JA Steinkamp

JE Aubin

JM McCord

JT Coyle

K Żamojć

L Hua Long

M Neumann

MAF Holleman

P Ubezio

S Alberti

S Reuter

SG Rhee

SW Yoon

VG Grivennikova

VL Mosiman

W Rao

Scientific Reports

AbstractWe have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C2O4)(pm)(OH2)2]+ (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.</jats:p

Dagmara Jacewicz

Kamila Siedlecka-Kroplewska

Joanna Drzeżdżon

Agnieszka Piotrowska

Dariusz Wyrzykowski

Aleksandra Tesmar

Krzysztof Żamojć

Lech Chmurzyński

Crossref

Method for detection of hydrogen peroxide in HT22 cells

Method for detection of hydrogen peroxide in HT22 cells

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