The aim of this study was to evaluate the shear bond strength (SBS) of two universal adhesives (Universal Single Bond and All Bond Universal) and a two-step self-etch adhesive system (Clearfil SE Bond) to dentine at various temperatures. Materials and Methods: One hundred and twenty dentin specimens were divided randomly to 12 groups, according to adhesive systems (Universal Single Bond and All Bond Universal, Clearfil SE Bond) and temperature ( 4ºC, 20 ºC, 36ºC, 55ºC) used. Dentin specimens were prepared (n :10, adhesives were applied, and composite cylinders were polymerized. Statistical analysis of the SBS data was performed using Two-way analysis of variance (ANOVA) and Tukey’s Honestly Significant Differences post-hoc test. Results: The Clearfil SE Bond was shown to have higher SBS than the universal adhesives at all temperatures; however, there was no statistically significant difference (P>0.05). In both groups, the lowest SBS values were observed in the samples at 4°C while the highest SBS values were observed in the samples at 55°C. In this case, there was a statistically significant difference (P 0.05). En todos los grupos, los valores más bajos de SBS se observaron en las muestras a 4°C, mientras que los valores de SBS más altos fueron obtenidos en las muestras a 55°C (P <0.05). Conclusiones: Los resultados sugieren que la efectividad de un adhesivo puede aumentar si se precalienta a 36°C o superior, antes de usarlo inmediatamente después de sacarlo del refrigerador o a temperatura ambiente.