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Development of anonymous nuclear markers from Illumina paired-end data for Seychelles caecilian amphibians (Gymnophiona: Indotyphlidae)
Authors
JJ Day
PG Foster
+6 more
DJ Gower
CJ Lewis
ST Maddock
C Morel
RA Nussbaum
M Wilkinson
Publication date
1 January 2014
Publisher
Doi
Cite
Abstract
Anonymous nuclear markers were developed for Seychelles caecilian amphibians. Using a previously published bioinformatics pipeline (developed for Roche 454 data), 36 candidate anonymous nuclear loci (ANL) of at least 180 bp length were identified from Illumina MiSeq next generation sequencing data for five Seychelles species. We designed primer pairs for the 36 candidate ANL and tested these by PCR and Sanger sequencing. Seven ANL amplified and sequenced well for at least five of the six nominal Seychelles caecilian species (in three genera), and represent potentially useful markers for systematics and conservation. © 2014 Springer Science+Business Media Dordrecht
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UCL Discovery
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oai:eprints.ucl.ac.uk.OAI2:141...
Last time updated on 28/12/2013
Springer - Publisher Connector
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Last time updated on 28/04/2017