The aim of this study was to prepare L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles which can
be used for the adsorption of IgG from aqueous solutions. L-lysine was complexed with MAAsp and Llysine-
imprinted poly(HEMA-MAAsp) nanoparticles were synthesized by miniemulsion polymerization. Also,
non-imprinted nanoparticles were synthesized without L-lysine for control purpose. L-lysine-imprinted
poly(HEMA-MAAsp) nanoparticles were characterized by means of elemental analysis, Fourier transform
infrared spectroscopy (FTIR) and transmission electron microscopy (TEM).
When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3500

Armutcu, C.

Corman, M.E.

Denizli, A.

Say, R.

Uzun, L.

SocioEconomic Challenges Journal (Sumy State University)

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE NANOMATERIALS: APPLICATIONS AND PROPERTIES 
Vol. 1 No 2, 02NNBM21(2pp) (2012) 
 
 
2304-1862/2012/1(2)02NNBM21(2) 02NNBM21-1  2012 Sumy State University 
L-Lysine Imprinted Nanoparticles for Antibody Biorecognition 
 
M. Emin Corman1, C. Armutcu1, L. Uzun1, R. Say2, A. Denizli1 
 
1 Hacettepe University, Department of Chemistry, Ankara, Turkey 
2 Anadolu University, Department of Chemistry, Eskisehir, Turkey  
 
(Received 18 June 2012; published online 22 August 2012) 
 
The aim of this study was to prepare L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles  which can 
be used for the adsorption of IgG from aqueous solutions. L-lysine was complexed with MAAsp and L-
lysine-imprinted poly(HEMA-MAAsp) nanoparticles were synthesized by miniemulsion polymerization. Al-
so, non-imprinted nanoparticles were synthesized without L-lysine for control purpose. L-lysine-imprinted 
poly(HEMA-MAAsp) nanoparticles were characterized by means of elemental analysis, Fourier transform 
infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). 
 
Keywords: Molecular imprinting, Nanoparticles, Poly(HEMA), Aspartic acid.  
 
  PACS numbers: 87.85.J – , 61.46.Df 
 
 
1. INTRODUCTION 
 
Advances in nanobiotechnology have resulted in the 
several novel colloidal carriers such as nanocomposite, 
nanorobots, nanocrystals, nanoparticles, killing effect 
during the tumor growth phase, and to protect the sur-
rounding healthy cells from unwanted exposure to the 
excess cytotoxic agent. Polymeric nanoparticles are the 
most attractive colloidal carriers owing several merits 
such as the ease of purification and sterilization, drug 
targeting possibility, and sustained release action[1].  
Molecularly imprinting is a novel technique to cre-
ate recognition sites for target molecule. Molecularly 
imprinted polymers show high selectivity for a target 
molecule[2]. Functional monomers form a complex with 
the template via covalent or noncovalent interactions 
and then in the presence of cross linking functional 
monomers  are polymerized.  After polymerization, the 
template is removed, and specific cavities that comple-
mentary to the template in size, shape, and position of 
the functional groups in the polymeric matrix are oc-
cured. Molecularly imprinted polymers (MIP) are easy 
to prepare, stable, inexpensive and capable of molecu-
lar recognition. Molecular recognition-based separation 
techniques have received much attention in various 
fields because of their high selectivity for target mole-
cules. One of the many attractive features of the mo-
lecular imprinting method is that it can be applied to a 
diverse range of analytes [3]. The imprinting of small, 
organic molecules (e.g., pharmaceuticals, pesticides, 
amino acids and peptides, nucleotide bases, steroids, 
and sugars) is succesfully reported. Somewhat larger 
organic compounds (e.g., peptides) can also be imprint-
ed via similar approaches, whereas the imprinting of 
much larger structures is still a challenge. If a short 
peptide or amino acid residue (lysine) representing only 
a small exposed fragment of a protein structure is used 
as a template, then the resultant macroporous MIP 
recognizing the imprinted peptide or amino acid will 
also be able to recognize the protein molecule. 
 
 
 
 
2. EXPERIMENTAL 
 
2.1 Materials 
 
Template molecule L-lysine immunoglobulin 
G (IgG), albumin (human serum), hemoglobulin, 
poly(vinyl alcohol) (PVA), sodium dodecyl sulfate 
(SDS), ammonium persulfate, sodium bicarbonate and 
sodium bisulfite were obtained from Sigma Chemical 
Co. (St. Louis, USA). Ethylene glycol dimethacrylate 
was purchased from Fluka A.G. (Buchs, Switzerland). 
All other chemicals were of reagent grade and pur-
chased from Merck A.G. (Darmstadt, Germany). 
 
2.2 Synthesis of N-methacryloyl-l-aspartic acid 
(MAAsp monomer) 
 
N-methacryloyl-L-aspartic acid (MAAsp) was cho-
sen as a pseudospecific ligand and synthesized by using 
methacryloyl chloride and L-aspartic acid [4]. 
 
2.3 Preparation of L-lysine imprinted poly-
(HEMA-MAAsp) nanoparticles 
 
L-lysine imprinted poly(HEMA-MAAsp) nanoparti-
cles were prepared by two-phase mini-emulsion 
polymerization method. The first aqueous phase was 
prepared by dissolving of PVA (200 mg), SDS (30 mg) 
and sodium bicarbonate (25 mg) in 10 mL deionized 
water. The second phase was prepared by dissolving of 
PVA (100 mg) and SDS (100 mg) in 200 mL of deion-
ized water. Functional monomer [MAAsp, 25 mg was 
dissolved in monomer (ethylene glycol dimethacrylate, 
2.1 mL) to form oil phase. The oil phase was slowly 
added to the first aqueous phase. In order to obtain 
mini-emulsion, the mixture was homogenized at 
25 000 rpm by a homogenizer (T10, Ika Labortechnik, 
Germany). After homogenization, the template mole-
cule [L-lysine, 22.7 mg was added to mini-emulsion to 
establish the ratio between monomer and template as 
1:1 in mole basis. Then, the mixture was slowly added 
to the second aqueous phase while the phase has been 
stirring in a sealed-cylindrical polymerization reactor 
(250 mL). The reactor was magnetically stirred at 
300 rpm (Radleys Carousel 6, Essex, UK). The 
 M. EMIN CORMAN, C. ARMUTCU, L. UZUN, R. SAY, A. DENIZLI PROC. NAP 1, 02NNBM21 (2012) 
 
 
02NNBM21-2 
polymerization mixture was slowly heated to 40°C, 
polymerization temperature. After that, nitrogen gas 
was bubbled through solution for 5 min to remove dis-
solved oxygen. Then, initiators, sodium bisulfite (125 
mg) and ammonium persulfate (125 mg), were added 
into the solution. Polymerization was continued for 
24 h. The obtained L-lysine imprinted nanoparticles 
were washed with water and water/ethyl alcohol mix-
tures, in order to remove unreacted monomers, surfac-
tant and initiator. The solutions were centrifuged at 30 
000 rpm for 30 min (Allegra-64R Beckman Coulter, 
USA) for each washing step and then the nanoparticles 
were dispersed in fresh solution. After last washing 
step, the nanoparticles were dispersed in deionized 
water containing 0.5% sodium azide to prevent contam-
ination and stored at 4°C. The non-imprinted nanopar-
ticles were synthesized by applying same procedure 
except addition of template molecules, L-lysine. 
 
2.4 Characterization of L-lysine imprinted poly- 
(HEMA-MAAsp) nanoparticles 
 
Nanoparticles were characterized by Zetasizer 
(NanoS, Malvern Instruments, London, UK), FTIR and 
TEM (FEI, Tecnai G2 F30, Oregon, USA). In zeta-size 
measurement, the light scattering was carried out at 
incidence angle 90° and 25°C.  For TEM analysis, im-
printed nanoparticle sample was dropped onto carbon 
coated copper grid and then dried at room temperature. 
TEM photographs were taken at 200 kV by TEM mi-
croscope. 
 
2.5 Adsorption of IgG on poly (HEMA-MAAsp) 
nanoparticles 
 
Adsorption of IgG on the oly (HEMA-MAAsp) nano-
particles from aqueous solutions was investigated 
batch-wise. The adsorption experiments were carried 
out at 25°C at stirring rate as 100 rpm for 2 h. Effects 
of pH of the medium, IgG concentration, temperature, 
salt type and ionic strength on the ad sorption capacity 
were studied. 
 
3. RESULTS AND DISCUSSION 
 
In this study, we present a simple polymerization 
method for preparing non-covalent molecularly im-
printed polymer (MIP) with specific IgG recognition 
sites. We have employed the surface-imprinting tech-
nique that relies on electrostatic interactions between a 
functional monomer and the chosen template molecule 
L-lysine to produce MIP capable of selective recognition 
in aqueous media. For this purpose, first, we prepared 
N-methacryloyl-(L)-aspartic acid (MAAsp) as a func-
tional monomer. Then, the L-lysine-imprinted, poly-
(hydroxylethyl methacrylate-N-methacryloyl-(L)-aspar-
tic acid methylester) [poly(HEMA-MAAsp)] nanoparti-
cles were synthesized via mini-emulsion polymeriza-
tion. Adsorption of IgG onto L-lysine-imprinted poly-
(HEMA-MAAsp) nanoparticles were investigated in 
batch system under various medium conditions (i.e. 
pH, ionic strength, IgG concentration, temperature). 
The results show that the imprinted nanoparticle has 
high selectivity and sensitivity for IgG. IgG adsorption 
capacity and molecular recognition selectivity studies 
in a batch system versus other proteins such as human 
serum albumin (HSA) and hemoglobin (Hb) were inves-
tigated and characterized in detail. Characterization of 
nanoparticles was conducted using FTIR, TEM, zeta 
sizer and elemental analysis. The specific surface area 
of the L-lysine-imprinted particles was found to be 
1872 m2/g with a size range of 110 nm in diameter. 
Finally, the reusability of the L-lysine-imprinted 
poly(HEMA-MAAsp) were evaluated there is no signifi-
cant loss in adsorption capacity after ten adsorption-
desorption cycles. 
 
 
REFERENCES 
 
1. A.E. David, N.S. Wang, V.C. Yang, A.J. Yang, J. Biotech-
nol. 125, 395 (2006). 
2. B. Sellergren, Anal. Chem. 66, 1578 (1994). 
G. Erturk, L. Uzun, M.A. Tumer, R. Say, A. Denizli, Bio-
sens. Bioelectron. 28, 97 (2011). 
3. D. Hür, S.F. Ekti, R. Say, Lett. Org. Chem. 4, 585 (2007).  
 


L-Lysine Imprinted Nanoparticles for Antibody Biorecognition

Electronic Sumy State University Institutional Repository

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE NANOMATERIALS: APPLICATIONS AND PROPERTIES Vol. 1 No 2, 02NNBM21(2pp) (2012)   2304-1862/2012/1(2)02NNBM21(2) 02NNBM21-1  2012 Sumy State University L-Lysine Imprinted Nanoparticles for Antibody Biorecognition  M. Emin Corman1, C. Armutcu1, L. Uzun1, R. Say2, A. Denizli1  1 Hacettepe University, Department of Chemistry, Ankara, Turkey 2 Anadolu University, Department of Chemistry, Eskisehir, Turkey   (Received 18 June 2012; published online 22 August 2012)  The aim of this study was to prepare L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles  which can be used for the adsorption of IgG from aqueous solutions. L-lysine was complexed with MAAsp and L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles were synthesized by miniemulsion polymerization. Al-so, non-imprinted nanoparticles were synthesized without L-lysine for control purpose. L-lysine-imprinted poly(HEMA-MAAsp) nanoparticles were characterized by means of elemental analysis, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM).  Keywords: Molecular imprinting, Nanoparticles, Poly(HEMA), Aspartic acid.     PACS numbers: 87.85.J – , 61.46.Df   1. INTRODUCTION  Advances in nanobiotechnology have resulted in the several novel colloidal carriers such as nanocomposite, nanorobots, nanocrystals, nanoparticles, killing effect during the tumor growth phase, and to protect the sur-rounding healthy cells from unwanted exposure to the excess cytotoxic agent. Polymeric nanoparticles are the most attractive colloidal carriers owing several merits such as the ease of purification and sterilization, drug targeting possibility, and sustained release action[1].  Molecularly imprinting is a novel technique to cre-ate recognition sites for target molecule. Molecularly imprinted polymers show high selectivity for a target molecule[2]. Functional monomers form a complex with the template via covalent or noncovalent interactions and then in the presence of cross linking functional monomers  are polymerized.  After polymerization, the template is removed, and specific cavities that comple-mentary to the template in size, shape, and position of the functional groups in the polymeric matrix are oc-cured. Molecularly imprinted polymers (MIP) are easy to prepare, stable, inexpensive and capable of molecu-lar recognition. Molecular recognition-based separation techniques have received much attention in various fields because of their high selectivity for target mole-cules. One of the many attractive features of the mo-lecular imprinting method is that it can be applied to a diverse range of analytes [3]. The imprinting of small, organic molecules (e.g., pharmaceuticals, pesticides, amino acids and peptides, nucleotide bases, steroids, and sugars) is succesfully reported. Somewhat larger organic compounds (e.g., peptides) can also be imprint-ed via similar approaches, whereas the imprinting of much larger structures is still a challenge. If a short peptide or amino acid residue (lysine) representing only a small exposed fragment of a protein structure is used as a template, then the resultant macroporous MIP recognizing the imprinted peptide or amino acid will also be able to recognize the protein molecule.     2. EXPERIMENTAL  2.1 Materials  Template molecule L-lysine immunoglobulin G (IgG), albumin (human serum), hemoglobulin, poly(vinyl alcohol) (PVA), sodium dodecyl sulfate (SDS), ammonium persulfate, sodium bicarbonate and sodium bisulfite were obtained from Sigma Chemical Co. (St. Louis, USA). Ethylene glycol dimethacrylate was purchased from Fluka A.G. (Buchs, Switzerland). All other chemicals were of reagent grade and pur-chased from Merck A.G. (Darmstadt, Germany).  2.2 Synthesis of N-methacryloyl-l-aspartic acid (MAAsp monomer)  N-methacryloyl-L-aspartic acid (MAAsp) was cho-sen as a pseudospecific ligand and synthesized by using methacryloyl chloride and L-aspartic acid [4].  2.3 Preparation of L-lysine imprinted poly-(HEMA-MAAsp) nanoparticles  L-lysine imprinted poly(HEMA-MAAsp) nanoparti-cles were prepared by two-phase mini-emulsion polymerization method. The first aqueous phase was prepared by dissolving of PVA (200 mg), SDS (30 mg) and sodium bicarbonate (25 mg) in 10 mL deionized water. The second phase was prepared by dissolving of PVA (100 mg) and SDS (100 mg) in 200 mL of deion-ized water. Functional monomer [MAAsp, 25 mg was dissolved in monomer (ethylene glycol dimethacrylate, 2.1 mL) to form oil phase. The oil phase was slowly added to the first aqueous phase. In order to obtain mini-emulsion, the mixture was homogenized at 25 000 rpm by a homogenizer (T10, Ika Labortechnik, Germany). After homogenization, the template mole-cule [L-lysine, 22.7 mg was added to mini-emulsion to establish the ratio between monomer and template as 1:1 in mole basis. Then, the mixture was slowly added to the second aqueous phase while the phase has been stirring in a sealed-cylindrical polymerization reactor (250 mL). The reactor was magnetically stirred at 300 rpm (Radleys Carousel 6, Essex, UK). The  M. EMIN CORMAN, C. ARMUTCU, L. UZUN, R. SAY, A. DENIZLI PROC. NAP 1, 02NNBM21 (2012)   02NNBM21-2 polymerization mixture was slowly heated to 40°C, polymerization temperature. After that, nitrogen gas was bubbled through solution for 5 min to remove dis-solved oxygen. Then, initiators, sodium bisulfite (125 mg) and ammonium persulfate (125 mg), were added into the solution. Polymerization was continued for 24 h. The obtained L-lysine imprinted nanoparticles were washed with water and water/ethyl alcohol mix-tures, in order to remove unreacted monomers, surfac-tant and initiator. The solutions were centrifuged at 30 000 rpm for 30 min (Allegra-64R Beckman Coulter, USA) for each washing step and then the nanoparticles were dispersed in fresh solution. After last washing step, the nanoparticles were dispersed in deionized water containing 0.5% sodium azide to prevent contam-ination and stored at 4°C. The non-imprinted nanopar-ticles were synthesized by applying same procedure except addition of template molecules, L-lysine.  2.4 Characterization of L-lysine imprinted poly- (HEMA-MAAsp) nanoparticles  Nanoparticles were characterized by Zetasizer (NanoS, Malvern Instruments, London, UK), FTIR and TEM (FEI, Tecnai G2 F30, Oregon, USA). In zeta-size measurement, the light scattering was carried out at incidence angle 90° and 25°C.  For TEM analysis, im-printed nanoparticle sample was dropped onto carbon coated copper grid and then dried at room temperature. TEM photographs were taken at 200 kV by TEM mi-croscope.  2.5 Adsorption of IgG on poly (HEMA-MAAsp) nanoparticles  Adsorption of IgG on the oly (HEMA-MAAsp) nano-particles from aqueous solutions was investigated batch-wise. The adsorption experiments were carried out at 25°C at stirring rate as 100 rpm for 2 h. Effects of pH of the medium, IgG concentration, temperature, salt type and ionic strength on the ad sorption capacity were studied.  3. RESULTS AND DISCUSSION  In this study, we present a simple polymerization method for preparing non-covalent molecularly im-printed polymer (MIP) with specific IgG recognition sites. We have employed the surface-imprinting tech-nique that relies on electrostatic interactions between a functional monomer and the chosen template molecule L-lysine to produce MIP capable of selective recognition in aqueous media. For this purpose, first, we prepared N-methacryloyl-(L)-aspartic acid (MAAsp) as a func-tional monomer. Then, the L-lysine-imprinted, poly-(hydroxylethyl methacrylate-N-methacryloyl-(L)-aspar-tic acid methylester) [poly(HEMA-MAAsp)] nanoparti-cles were synthesized via mini-emulsion polymeriza-tion. Adsorption of IgG onto L-lysine-imprinted poly-(HEMA-MAAsp) nanoparticles were investigated in batch system under various medium conditions (i.e. pH, ionic strength, IgG concentration, temperature). The results show that the imprinted nanoparticle has high selectivity and sensitivity for IgG. IgG adsorption capacity and molecular recognition selectivity studies in a batch system versus other proteins such as human serum albumin (HSA) and hemoglobin (Hb) were inves-tigated and characterized in detail. Characterization of nanoparticles was conducted using FTIR, TEM, zeta sizer and elemental analysis. The specific surface area of the L-lysine-imprinted particles was found to be 1872 m2/g with a size range of 110 nm in diameter. Finally, the reusability of the L-lysine-imprinted poly(HEMA-MAAsp) were evaluated there is no signifi-cant loss in adsorption capacity after ten adsorption-desorption cycles.   REFERENCES  1. A.E. David, N.S. Wang, V.C. Yang, A.J. Yang, J. Biotech-nol. 125, 395 (2006). 2. B. Sellergren, Anal. Chem. 66, 1578 (1994). G. Erturk, L. Uzun, M.A. Tumer, R. Say, A. Denizli, Bio-sens. Bioelectron. 28, 97 (2011). 3. D. Hür, S.F. Ekti, R. Say, Lett. Org. Chem. 4, 585 (2007).   

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L-Lysine Imprinted Nanoparticles for Antibody Biorecognition

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