Albuterol, which increases the muscle mass and decreases the adipose tissue, is misused as nutrient
repartitioning agent in livestock. Hence, a new sensitive method for determination of albuterol in livestock
is presented. Four nano-baskets of calix[4]crown were synthesized and used to prepare nano-based bendedphases
of HPLC–UV. The new synthesized bonded-phases were characterized and optimized, the bonding
interactions of solute:stationary phases were examined and the main interactions were reported. The albuterol
level of six samples of livestock meat including pork, pork casing, beef, beef casing, mutton, and
mutton casing were analyzed and the results revealed that for the best bonded-phases, the LOD and LOQ
were 0.06 and 0.20 μg/mL, respectively.
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