Bokma, Jade

Boyen, Filip

De Keersmaecker, Sigrid

Pardon, Bart

Pas, Mathilde

Roosens, Nancy

Van Driessche, Laura

Vanneste, Kevin

English

Ghent University Academic Bibliography

NEONATAL SEPSIS CAUSED BY RAOULTELLA ORNITHINOLYTICA IN A CALFDEPARTMENT OF LARGE ANIMAL INTERNAL MEDICINEMathilde L. Pas1, Jade Bokma1, Laura Van Driessche1, Kevin Vanneste2, Sigrid De Keersmaecker2, Nancy Roosens2, Filip Boyen3, Bart Pardon1Contactmathilde.pas@ugent.beConclusionIntroductionCase presentation1 Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Belgium2 Sciensano, Elsene, Belgium 3 Department of Pathology, Bacteriology and Poultry diseases, Faculty of Veterinary Medicine, Ghent UniversityAs in humans, multidrug resistant Raoultella ornithinolytica strains can cause septicaemia incalves. Whether R. ornithinolytica should be considered as a hospital-acquired or on farm infectionremains to be determined. Therapeutic failure in such multidrug resistant strains might occur.Rapid antibiotic susceptibility testing can increase therapeutic efficiency in similar cases.Table 1: Antibiotic resistance genes possibly linked with observed phenotypic resistance in Raoultellaornithinolytica strain obtained from a calf with septicaemia.Raoultella ornithinolytica is an encapsulated, aerobic, Gram-negativebacillus, formerly classified as a Klebsiella species. Bacteraemia with thisbacterium has been described in humans in various reports. This is a firstcase describing Raoultella ornithinolytica septicaemia in cattle.A Belgian Blue, ♂, 2 days old calf was presented December 2018. Clinicalsigns were dyspnoea, pyrexia (40,8°C) and lateral decubitus. Lungultrasound showed diffuse comet tails and multiple small consolidations.Arterial oxygen pressure was 32 mmHg and hypercapnia was present.Failure of passive transfer was excluded with a glutaraldehyde test. Empirictreatment with enrofloxacin was successful.Laboratory analysisBlood culture samples (BD BACTEC™) were taken sterile out of thejugular vein, which resulted in the isolation of a pure culture of agram negative bacterium (figure 1). Identification of the isolatewas performed with MALDI-TOF MS (Bruker Daltonik GmbH,Bremen, Germany). The best hit was obtained with Raoultellaornithinolytica MB_18887 CHB with a score value of 2.32.Antimicrobial susceptibility testing was determined with the diskdiffusing test, showing that the isolate was multidrug resistant(table 1). Whole genome sequencing (Illumina MiSeq) was used toconfirm identification and identify antimicrobial resistance genes(table 1). The combination of 16S analysis, k-mer based analysisand read mapping analysis highly supported classification asRaoultella ornithinolytica. In addition, nine different antimicrobialresistance genes were detected, including the plasmid-mediatedquinolone resistance (PMQR) gene qnrS1.SusceptibiltyBLAST-based detection AMR geneslocus % identityAmoxicillin RESISTENTblaPLA1a 94,41blaTEM-1B 100,00Amoxicillin + Clavulanic acidIntermediateblaPLA1a 94,41blaTEM-1B 100,00Apramicin SusceptibleCephalexin SusceptibleCefquinome SusceptibleCeftiofur SusceptibleDoxycycline RESISTENT tet(D) 100,00Enrofloxacin Susceptible qnrS1 100,00Florfenicol SusceptibleFlumequine RESISTENT qnrS1 100,00Gentamicin RESISTENT aac(3)-IIa 100,00Kanamycin Susceptible aph(3")-Ib 99,88Marbofloxacin Susceptible qnrS1 100,00Paromomycin SusceptibleSpectinomycin SusceptibleSulfa-trimethoprim RESISTENTsul2 100,00dfrA14 100,00Fosfomycin Not tested FosA 99,52Figure 1: Pure culture of Raoultella ornithinolytica acquired from sepsis-suspected calf.

Neonatal sepsis caused by Raoultella ornithinolytica in a calf

https://biblio.ugent.be/publication/8631539/file/8631547

Neonatal sepsis caused by Raoultella ornithinolytica in a calf

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