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Rapid Differentiation between Livestock-Associated and Livestock-Independent Staphylococcus aureus CC398 Clades
Authors
A van Belkum
AC Uhlemann
+45 more
AC Uhlemann
AC Uhlemann
AJ McCarthy
Alex van Belkum
Anders R. Larsen
Andreas Petersen
BAGL van Cleef
BV Lowder
C Cuny
C Mammina
C Mammina
Caterina Mammina
Cindy M. Liu
F Yu
Frederic Laurent
G Resch
H Graveland
HC Lewis
Jesper Larsen
JN Jimenez
JP Rasigade
JR Mediavilla
Judy N. Jiménez
Katerina Soldanova
Lance B. Price
LB Price
LE Spoor
M Bhat
Maliha Aziz
Marc Stegger
Michael Otto
MW Wassenberg
N van der Mee-Marquet
Paal S. Andersen
PR Davies
R Ruimy
Robert L. Skov
S Salmenlinna
S Vandendriessche
Saara Salmenlinna
SHM Rooijakkers
Stien Vandendriessche
Tania Contente-Cuomo
UC Warsa
WJ van Wamel
Publication date
1 January 2013
Publisher
'Public Library of Science (PLoS)'
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Abstract
Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on single-nucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet (M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies. © 2013 Stegger et al.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
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