A Matusch

A Mencacci

AE Armitage

Alistair J. P. Brown

AM Thomson

Andrea Raab

AS Ibrahim

B Spellberg

BD Corbin

CH Park

CJ Barelle

CM Lloyd

CP Smith

D Kaloriti

D Suckau

Dagmar S. Urgast

David Stead

DD Haines

DH Navarathna

DM Barry

DM MacCallum

Donna M. MacCallum

DS Urgast

ED Weinberg

EE Johnson

EH Seeley

G Kovtunovych

GD Brown

GO Latunde-Dada

H Drakesmith

H Haas

H Wang

HI Feldman

IV Ene

JC Sardi

JF McManus

JM El HageChahine

Joanna Potrykus

Jörg Feldmann

M Ogawa

MC Lorenz

MD Maines

MI Hood

MJ Marakalala

ML Pendrak

N Ramanan

N Van Rooijen

NA Gow

Nico van Rooijen

P Heymann

Q Qian

R Santos

RJ Ward

RS Almeida

S Shimma

SA Knight

Scott G. Filler

T Roemer

W Fonzi

WH Jung

PLoS Pathogens

English

PubMed

<div><p>Nutritional immunity – the withholding of nutrients by the host – has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS), MALDI imaging and immunohistochemistry with microtranscriptomics to examine iron homeostasis in the host and pathogen in the murine model of systemic candidiasis. Dramatic changes in the renal iron landscape occur during disease progression. The infection perturbs global iron homeostasis in the host leading to iron accumulation in the renal medulla. Paradoxically, this is accompanied by nutritional immunity in the renal cortex as iron exclusion zones emerge locally around fungal lesions. These exclusion zones correlate with immune infiltrates and haem oxygenase 1-expressing host cells. This local nutritional immunity decreases iron availability, leading to a switch in iron acquisition mechanisms within mature fungal lesions, as revealed by laser capture microdissection and qRT-PCR analyses. Therefore, a complex interplay of systemic and local events influences iron homeostasis and pathogen-host dynamics during disease progression.</p></div

Joanna Potrykus (404374)

David Stead (473378)

Donna M. MacCallum (193793)

Dagmar S. Urgast (473379)

Andrea Raab (249766)

Nico van Rooijen (4110211)

Jörg Feldmann (473380)

Alistair J. P. Brown (108565)

The Francis Crick Institute

Fungal Iron Availability during Deep Seated Candidiasis Is Defined by a Complex Interplay Involving Systemic and Local Events

Nutritional immunity--the withholding of nutrients by the host--has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS), MALDI imaging and immunohistochemistry with microtranscriptomics to examine iron homeostasis in the host and pathogen in the murine model of systemic candidiasis. Dramatic changes in the renal iron landscape occur during disease progression. The infection perturbs global iron homeostasis in the host leading to iron accumulation in the renal medulla. Paradoxically, this is accompanied by nutritional immunity in the renal cortex as iron exclusion zones emerge locally around fungal lesions. These exclusion zones correlate with immune infiltrates and haem oxygenase 1-expressing host cells. This local nutritional immunity decreases iron availability, leading to a switch in iron acquisition mechanisms within mature fungal lesions, as revealed by laser capture microdissection and qRT-PCR analyses. Therefore, a complex interplay of systemic and local events influences iron homeostasis and pathogen-host dynamics during disease progression

Donna M Maccallum

Dagmar S Urgast

Alistair J P Brown

Directory of Open Access Journals

Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.

Potrykus, J.

Stead, D.

MacCallum, D.M.

Urgast, D.S.

Raab, A.

van Rooijen, N.

Feldmann, J.

Brown, A.J.P.

NARCIS 

Crossref

Potrykus Joanna

Stead David

MacCallum Donna M.

Urgast Dagmar S.

Raab Andrea

van Rooijen Nico

Feldmann Jörg

Brown Alistair J. P.

Public Library of Science (PLOS)

Nutritional immunity – the withholding of nutrients by the host – has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS), MALDI imaging and immunohistochemistry with microtranscriptomics to examine iron homeostasis in the host and pathogen in the murine model of systemic candidiasis. Dramatic changes in the renal iron landscape occur during disease progression. The infection perturbs global iron homeostasis in the host leading to iron accumulation in the renal medulla. Paradoxically, this is accompanied by nutritional immunity in the renal cortex as iron exclusion zones emerge locally around fungal lesions. These exclusion zones correlate with immune infiltrates and haem oxygenase 1-expressing host cells. This local nutritional immunity decreases iron availability, leading to a switch in iron acquisition mechanisms within mature fungal lesions, as revealed by laser capture microdissection and qRT-PCR analyses. Therefore, a complex interplay o

Donna M. Maccallum

Nico Van Rooijen

CiteSeerX

Potrykus, Joanna

Stead, David

Maccallum, Donna M

Urgast, Dagmar S

Raab, Andrea

van Rooijen, Nico

Feldmann, Jörg

Brown, Alistair J P

Aberdeen University Research

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Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events

https://aura.abdn.ac.uk/bitstream/2164/3052/1/potrykus_plospathogen.pdf

Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events

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Aberdeen University Research