Ice-nucleating particles (INPs) are atmospheric aerosol particles that can strongly influence the radiative properties and precipitation onset in mixed-phase clouds by triggering ice formation in supercooled cloud water droplets. The ability to distinguish between INPs of mineral and biological origin in samples collected from the environment is needed to better understand their distribution and sources. A common method for assessing the relative contributions of mineral and biogenic INPs in samples collected from the environment (e.g. aerosol, rainwater, soil) is to determine the ice-nucleating ability (INA) before and after heating, where heat is expected to denature proteins associated with some biological ice nucleants. The key assumption is that the ice nucleation sites of biological origin are denatured by heat, while those associated with mineral surfaces remain unaffected; we test this assumption here. We exposed atmospherically relevant mineral samples to wet heat (INP suspensions warmed to above 90 ∘C) or dry heat (dry samples heated up to 250 ∘C) and assessed the effects on their immersion mode INA using a droplet freezing assay. K-feldspar, thought to be the dominant mineral-based atmospheric INP type where present, was not significantly affected by wet heating, while quartz, plagioclase feldspars and Arizona Test Dust (ATD) lost INA when heated in this mode. We argue that these reductions in INA in the aqueous phase result from direct alteration of the mineral particle surfaces by heat treatment rather than from biological or organic contamination. We hypothesise that degradation of active sites by dissolution of mineral surfaces is the mechanism in all cases due to the correlation between mineral INA deactivation magnitudes and their dissolution rates. Dry heating produced minor but repeatable deactivations in K-feldspar particles but was generally less likely to deactivate minerals compared to wet heating. We also heat-tested biogenic INP proxy materials and found that cellulose and pollen washings were relatively resistant to wet heat. In contrast, bacterially and fungally derived ice-nucleating samples were highly sensitive to wet heat as expected, although their activity remained non-negligible after wet heating. Dry heating at 250 ∘C leads to deactivation of all biogenic INPs. However, the use of dry heat at 250 ∘C for the detection of biological INPs is limited since K-feldspar's activity is also reduced under these conditions. Future work should focus on finding a set of dry heat conditions where all biological material is deactivated, but key mineral types are not. We conclude that, while wet INP heat tests at (>90 ∘C) have the potential to produce false positives, i.e. deactivation of a mineral INA that could be misconstrued as the presence of biogenic INPs, they are still a valid method for qualitatively detecting very heat-sensitive biogenic INPs in ambient samples if the mineral-based INA is controlled by K-feldspar