Vrijednost citološke analize, DNA kvantifikacije protočnom citometrijom i određivanja ekspresije topoizomeraze II-α u procjeni stupnja displazije sluznice debelog crijeva
Patohistologycal analysis of colon mucosa biopsy is a golden standard in diagnosing malignant and premalignant lesions.
In this work additional methods: cytological analysis of imprint smear of colon mucosa biopsy, expression of topoisomerase II alpha and ploidy determination, have been analized as adjunct to the patohystological analysis in achieving better distinction in some borderline cases of dysplasia and malignant alteration.
According to data there is significantly higher expression of topoisomerase II alpha in malignant lesions of colonic mucosa comparing to dysplasia and inflammation in colonic mucosa. There is also significant difference in topoisomerase II alpha expression between low and high-grade dysplasia as well as between low and high-grade tumors.
Significant difference in ploidy of malignant lesions comparing to dysplasia and inflammation in colonic mucosa is also found, as well as difference between different dysplasia grades.
This data show that those two methods (determination of topoisomerase II alpha expression, and determination of ploidy), especially when they are combined, are valuable help for standard patohystological analysis.
Cytological analysis of biopsy samples of colonic mucosa is sensitive and specific for detecting malignant lesions, but is insufficient in determining dysplasia, inflammation and adenoma type. It can be used as complementary method with patohystological analysis.
DNA flow cytometry, which was used to determine sample ploidy, has also shown the value of S-faze determination, wich could be helpful in diagnostic algorithm of diagnosing dysplasia in colonic mucosa, especially in inflammatory bowel disease, but other investigations are needed since our sample size was not big enough, and there wasn’t any analysed samples of inflammatory bowel disease with high grade dysplasia
