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Liu, Mengcheng

Logan, Hannah

Newman, Jamie

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Louisiana Tech Digital Commons

Louisiana Tech UniversityLouisiana Tech Digital CommonsANS Research Symposium ANS Research Symposium 2019Apr 11th, 8:30 AM - 11:30 AMThe Role of Notch1 and Notch3 in hADSCAdipogenesisMengcheng LiuLouisiana Tech UniversityHannah LoganLouisiana Tech UniversityJamie NewmanLouisiana Tech UniversityFollow this and additional works at: https://digitalcommons.latech.edu/ans-research-symposiumThis Event is brought to you for free and open access by the Conferences and Symposia at Louisiana Tech Digital Commons. It has been accepted forinclusion in ANS Research Symposium by an authorized administrator of Louisiana Tech Digital Commons. For more information, please contactdigitalcommons@latech.edu.Recommended CitationLiu, Mengcheng; Logan, Hannah; and Newman, Jamie, "The Role of Notch1 and Notch3 in hADSC Adipogenesis" (2019). ANSResearch Symposium. 30.https://digitalcommons.latech.edu/ans-research-symposium/2019/poster-presentations/30 45    The Role of Notch1 and Notch3 in hADSC Adipogenesis  Mengcheng Liu1, Hannah Logan2, and Jamie Newman3  1Graduate Student, School of Biological Sciences, Louisiana Tech University 2Undergraduate Student, School of Biological Sciences, Louisiana Tech University 3Assistant Professor, School of Biological Sciences, Louisiana Tech University  ____________________________________________________________________________________ Human adipose-derived stem cells (hADSCs) are multipotent cells with great potential in regenerative medicine. Notch is a conserved contact-dependent signaling pathway that determines cell proliferation and differentiation. The pathway includes four membrane receptors, Notch1-4, and our goal is to explore the role of Notch1 and Notch3 in hADSC adipogenesis to understand how they work together to regulate cell state. We found that the loss of Notch3 promoted adipogenesis as demonstrated by increased lipid droplets and enhanced expression of adipose-related genes. In addition, we observed increased expression of Notch3 during adipogenesis. Expression of Notch1 also increased during adipogenesis but, unlike Notch3, appears to promote lipid accumulation. Immunofluorescence showed that Notch1 was enriched in adipocyte differentiated hADSCs while Notch3 was highly expressed in and only in adjacent hADSCs not undergoing adipogenesis to maintain cells at stem cells state. Our research provides new targets for directed differentiation in tissue regeneration. Format: Poster presentation               

The Role of Notch1 and Notch3 in hADSC Adipogenesis

https://digitalcommons.latech.edu/cgi/viewcontent.cgi?article=1095&amp;context=ans-research-symposium

The Role of Notch1 and Notch3 in hADSC Adipogenesis

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